| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 100mg |
|
||
| 500mg |
| 动物实验 |
- Male rats were utilized for the study. Rats were fed diets supplemented with meats from different sources (beef, pork, chicken) or exogenous 3-methylhistidine. Urine samples were continuously collected for 24-72 hours post-feeding, acidified immediately, and stored at -20°C for subsequent analysis. 1-Methyl-L-histidine in urine was quantified using chromatographic techniques to verify its excretion as a marker of exogenous intake [1]
|
|---|---|
| 药代性质 (ADME/PK) |
- 1-Methyl-L-histidine is a urinary excretion metabolite serving as a qualitative indicator of exogenous 3-methylhistidine intake. After oral ingestion of exogenous 3-methylhistidine or meats from various sources (beef, pork, chicken), 1-Methyl-L-histidine is excreted in urine without significant metabolic transformation. It is detectable in urine within 24 hours of intake, and its presence specifically confirms the ingestion of exogenous 3-methylhistidine-containing proteins [1]
|
| 参考文献 | |
| 其他信息 |
N(tele)-methyl-L-histidine is a L-histidine derivative in which the methyl group is at N(tele)-position. It has a role as a human metabolite. It is a L-histidine derivative and a non-proteinogenic L-alpha-amino acid. It is a tautomer of a N(tele)-methyl-L-histidine zwitterion.
1-Methylhistidine is a metabolite found in or produced by Escherichia coli (strain K12, MG1655). 1-Methyl-L-histidine has been reported in Prunus domestica with data available. 1-Methylhistidine is a histidine derivative that has a methyl group bound to the nitrogen at position 1 and results from the metabolism of the dipeptide anserine. Urinary levels of 1-methylhistidine may be indicative of myofibrillar protein degradation or a high protein diet. - 1-Methyl-L-histidine is a naturally occurring methylated histidine derivative [1] - It is a specific qualitative biomarker for exogenous 3-methylhistidine intake and meat consumption (beef, pork, chicken), as it is not endogenously synthesized in mammals. Its urinary excretion directly reflects the ingestion of proteins rich in 3-methylhistidine [1] |
| 分子式 |
C7H11N3O2
|
|---|---|
| 分子量 |
169.18114
|
| 精确质量 |
169.085
|
| CAS号 |
332-80-9
|
| PubChem CID |
92105
|
| 外观&性状 |
White to off-white solid powder
|
| 密度 |
1.4±0.1 g/cm3
|
| 沸点 |
415.0±35.0 °C at 760 mmHg
|
| 熔点 |
~240 °C (dec.)(lit.)
|
| 闪点 |
204.8±25.9 °C
|
| 蒸汽压 |
0.0±1.0 mmHg at 25°C
|
| 折射率 |
1.613
|
| LogP |
-1.31
|
| tPSA |
81.14
|
| 氢键供体(HBD)数目 |
2
|
| 氢键受体(HBA)数目 |
4
|
| 可旋转键数目(RBC) |
3
|
| 重原子数目 |
12
|
| 分子复杂度/Complexity |
174
|
| 定义原子立体中心数目 |
1
|
| SMILES |
CN1C=C(N=C1)C[C@@H](C(=O)O)N
|
| InChi Key |
BRMWTNUJHUMWMS-LURJTMIESA-N
|
| InChi Code |
InChI=1S/C7H11N3O2/c1-10-3-5(9-4-10)2-6(8)7(11)12/h3-4,6H,2,8H2,1H3,(H,11,12)/t6-/m0/s1
|
| 化学名 |
(2S)-2-amino-3-(1-methylimidazol-4-yl)propanoic acid
|
| HS Tariff Code |
2934.99.9001
|
| 存储方式 |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| 运输条件 |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| 溶解度 (体外实验) |
H2O : ~125 mg/mL (~738.86 mM)
|
|---|---|
| 溶解度 (体内实验) |
配方 1 中的溶解度: 100 mg/mL (591.09 mM) in PBS (这些助溶剂从左到右依次添加，逐一添加), 澄清溶液; 超声助溶。
请根据您的实验动物和给药方式选择适当的溶解配方/方案： 1、请先配制澄清的储备液(如：用DMSO配置50 或 100 mg/mL母液(储备液)); 2、取适量母液，按从左到右的顺序依次添加助溶剂，澄清后再加入下一助溶剂。以 下列配方为例说明 (注意此配方只用于说明，并不一定代表此产品 的实际溶解配方): 10% DMSO → 40% PEG300 → 5% Tween-80 → 45% ddH2O (或 saline); 假设最终工作液的体积为 1 mL, 浓度为5 mg/mL: 取 100 μL 50 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀/澄清；向上述体系中加入50 μL Tween-80，混合均匀/澄清；然后继续加入450 μL ddH2O (或 saline)定容至 1 mL； 3、溶剂前显示的百分比是指该溶剂在最终溶液/工作液中的体积所占比例; 4、 如产品在配制过程中出现沉淀/析出，可通过加热(≤50℃)或超声的方式助溶; 5、为保证最佳实验结果，工作液请现配现用！ 6、如不确定怎么将母液配置成体内动物实验的工作液，请查看说明书或联系我们; 7、 以上所有助溶剂都可在 Invivochem.cn网站购买。 |
| 制备储备液 | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.9109 mL | 29.5543 mL | 59.1086 mL | |
| 5 mM | 1.1822 mL | 5.9109 mL | 11.8217 mL | |
| 10 mM | 0.5911 mL | 2.9554 mL | 5.9109 mL |
1、根据实验需要选择合适的溶剂配制储备液 (母液)：对于大多数产品，InvivoChem推荐用DMSO配置母液 (比如：5、10、20mM或者10、20、50 mg/mL浓度），个别水溶性高的产品可直接溶于水。产品在DMSO 、水或其他溶剂中的具体溶解度详见上”溶解度 (体外)”部分;
2、如果您找不到您想要的溶解度信息，或者很难将产品溶解在溶液中，请联系我们;
3、建议使用下列计算器进行相关计算（摩尔浓度计算器、稀释计算器、分子量计算器、重组计算器等）;
4、母液配好之后，将其分装到常规用量，并储存在-20°C或-80°C，尽量减少反复冻融循环。
计算结果:
工作液浓度： mg/mL；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL)。如该浓度超过该批次药物DMSO溶解度，请首先与我们联系。
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL ddH2O，混匀澄清。
(1) 请确保溶液澄清之后，再加入下一种溶剂 (助溶剂) 。可利用涡旋、超声或水浴加热等方法助溶；
(2) 一定要按顺序加入溶剂 (助溶剂) 。
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