Endogenous Metabolite

8-羟基-2'-脱氧鸟苷 (8-OHdG) 是一种有用的生物标志物，可用于确定患多种癌症和退行性疾病的风险。生物标志物 8-羟基-2'-脱氧鸟苷 (8-OHdG)，也称为 8-oxodG，参与癌发生的开始和进展，并已成为评估 DNA 内源性氧化损伤后果的关键标志物。在接触烟草烟雾、石棉纤维、重金属和多环芳烃等致癌物质后，该生物标记物已被用于评估人类 DNA 损伤[1]。

有大量的实验证据表明，细胞膜、蛋白质和DNA的脂质会永久发生氧化损伤。在核和线粒体DNA中，8-羟基-2'-脱氧鸟苷（8-OHdG）或8-氧代-7,8-二氢-2'-去氧鸟苷（8-氧代dG）是自由基诱导的氧化损伤的主要形式之一，因此被广泛用作氧化应激和致癌的生物标志物。研究表明，尿8-OHdG是评估各种癌症和退行性疾病风险的良好生物标志物。最广泛使用的定量分析方法是电化学检测（EC）的高效液相色谱（HPLC）、气相色谱-质谱（GC-MS）和HPLC串联质谱。为了解决定量测量8-OHdG时遇到的方法学问题，1997年成立了欧洲氧化DNA损伤标准委员会，以解决氧化DNA产物分离和纯化过程中的人为氧化问题。生物标志物8-OHdG或8-oxodG一直是测量内源性氧化损伤对DNA影响的关键标志物，也是启动和促进癌症发生的一个因素。该生物标志物已被用于评估人类在接触烟草烟雾、石棉纤维、重金属和多环芳烃等致癌物质后的DNA损伤。近年来，8-OHdG在许多研究中被广泛使用，不仅作为测量内源性氧化DNA损伤的生物标志物，而且作为包括癌症在内的许多疾病的危险因素[1]。

Toxicity Summary
Uremic toxins such as 8-Hydroxy-2Х-deoxyguanosine are actively transported into the kidneys via organic ion transporters (especially OAT3). Increased levels of uremic toxins can stimulate the production of reactive oxygen species. This seems to be mediated by the direct binding or inhibition by uremic toxins of the enzyme NADPH oxidase (especially NOX4 which is abundant in the kidneys and heart) (A7868). Reactive oxygen species can induce several different DNA methyltransferases (DNMTs) which are involved in the silencing of a protein known as KLOTHO. KLOTHO has been identified as having important roles in anti-aging, mineral metabolism, and vitamin D metabolism. A number of studies have indicated that KLOTHO mRNA and protein levels are reduced during acute or chronic kidney diseases in response to high local levels of reactive oxygen species (A7869).

[1]. 8-hydroxy-2' -deoxyguanosine (8-OHdG): A critical biomarker of oxidative stress and carcinogenesis. J Environ Sci Health C Environ Carcinog Ecotoxicol Rev. 2009 Apr;27(2):120-39.

8-hydroxy-2'-deoxyguanosine is guanosine substituted at the purine 8-position by a hydroxy group. It is used as a biomarker of oxidative DNA damage. It has a role as a biomarker.
8-Hydroxy-2'-deoxyguanosine has been reported in Vibrio harveyi with data available.
8-Hydroxy-2'-deoxyguanosine is a major spontaneous oxidized derivative of 2'-deoxyguanosine and a biomarker of oxidative DNA damage. 8-hydroxy-2'-deoxyguanosine (8-oxo-dG) is formed through the reaction of guanine with reactive oxygen species. Although normally repaired and removed by the base excision repair mechanism, 8-oxo-dG can potentially mispair with deoxyadenine leading to G-to-T transversion mutations, which cause frequent recombinations and single nucleotide polymorphisms (SNPs) in the human genome. The concentration of 8-oxo-dG within a cell is a measurement of oxidative stress and may thus be used to assess the extent of physiological and environmental damage to DNA.
Common oxidized form of deoxyguanosine in which C-8 position of guanine base has a carbonyl group.

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8-Hydroxy-deoxyguanosine is a uremic toxin. Uremic toxins can be subdivided into three major groups based upon their chemical and physical characteristics: 1) small, water-soluble, non-protein-bound compounds, such as urea; 2) small, lipid-soluble and/or protein-bound compounds, such as the phenols and 3) larger so-called middle-molecules, such as beta2-microglobulin. Chronic exposure of uremic toxins can lead to a number of conditions including renal damage, chronic kidney disease and cardiovascular disease. 8-Hydroxy-deoxyguanosine (8-OHdG) is a sensitive marker of the DNA damage due to hydroxyl radical attack at the C8 of guanine. This damage, if left unrepaired, has been proposed to contribute to mutagenicity and cancer promotion.
Common oxidized form of deoxyguanosine in which C-8 position of guanine base has a carbonyl group.

283.091

C, 42.41; H, 4.63; N, 24.73; O, 28.24

88847-89-6

8-Hydroxy-2'-deoxyguanosine-15N5;569649-11-2

135440064

White to off-white solid powder

2.3±0.1 g/cm3

217-220ºC

1.955

-1.32

159.51

5

6

2

20

544

3

C1[C@@H]([C@H](O[C@H]1N2C3=C(C(=O)NC(=N3)N)NC2=O)CO)O

HCAJQHYUCKICQH-VPENINKCSA-N

InChI=1S/C10H13N5O5/c11-9-13-7-6(8(18)14-9)12-10(19)15(7)5-1-3(17)4(2-16)20-5/h3-5,16-17H,1-2H2,(H,12,19)(H3,11,13,14,18)/t3-,4+,5+/m0/s1

2-amino-9-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-1,7-dihydropurine-6,8-dione

8 Oxo dG; 8-OHdG; 88847-89-6; 8-Hydroxy-2'-deoxyguanosine; 8-Oxo-2'-deoxyguanosine; 8-Oxo-dG; 8-Hydroxydeoxyguanosine; 8-Oxo-7,8-dihydro-2'-deoxyguanosine; 2'-deoxy-8-oxoguanosine; 8-Oxo-7-hydrodeoxyguanosine; 8 OHdG; 8-Oxo-dG

2934.99.9001

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

DMSO : ~155 mg/mL (~547.24 mM)

配方 1 中的溶解度: ≥ 2.58 mg/mL (9.11 mM) (饱和度未知) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (这些助溶剂从左到右依次添加，逐一添加), 澄清溶液。
例如，若需制备1 mL的工作液，可将100 μL 25.8 mg/mL澄清DMSO储备液加入400 μL PEG300中，混匀；然后向上述溶液中加入50 μL Tween-80，混匀；加入450 μL生理盐水定容至1 mL。
*生理盐水的制备：将 0.9 g 氯化钠溶解在 100 mL ddH₂O中，得到澄清溶液。

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配方 2 中的溶解度: ≥ 2.58 mg/mL (9.11 mM) (饱和度未知) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (这些助溶剂从左到右依次添加，逐一添加), 澄清溶液。
例如，若需制备1 mL的工作液，可将 100 μL 25.8mg/mL澄清的DMSO储备液加入到900μL 20％SBE-β-CD生理盐水中，混匀。
*20% SBE-β-CD 生理盐水溶液的制备（4°C，1 周）：将 2 g SBE-β-CD 溶解于 10 mL 生理盐水中，得到澄清溶液。

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配方 3 中的溶解度: ≥ 2.58 mg/mL (9.11 mM) (饱和度未知) in 10% DMSO + 90% Corn Oil (这些助溶剂从左到右依次添加，逐一添加), 澄清溶液。
例如，若需制备1 mL的工作液，可将 100 μL 25.8 mg/mL澄清DMSO储备液加入900 μL玉米油中，混合均匀。

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请根据您的实验动物和给药方式选择适当的溶解配方/方案：
1、请先配制澄清的储备液(如：用DMSO配置50 或 100 mg/mL母液(储备液));
2、取适量母液，按从左到右的顺序依次添加助溶剂，澄清后再加入下一助溶剂。以 下列配方为例说明 (注意此配方只用于说明，并不一定代表此产品 的实际溶解配方):
10% DMSO → 40% PEG300 → 5% Tween-80 → 45% ddH2O (或 saline);
假设最终工作液的体积为 1 mL, 浓度为5 mg/mL: 取 100 μL 50 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀/澄清；向上述体系中加入50 μL Tween-80，混合均匀/澄清；然后继续加入450 μL ddH2O (或 saline)定容至 1 mL；
3、溶剂前显示的百分比是指该溶剂在最终溶液/工作液中的体积所占比例;
4、 如产品在配制过程中出现沉淀/析出，可通过加热(≤50℃)或超声的方式助溶;
5、为保证最佳实验结果，工作液请现配现用！
6、如不确定怎么将母液配置成体内动物实验的工作液，请查看说明书或联系我们;
7、 以上所有助溶剂都可在 Invivochem.cn网站购买。