Indocyanine Green   中文名称: 吲哚菁绿

Fluorescent dye

通过浓度为 1000 μg/mL 的吲哚菁绿 (Foxgreen)-光动力疗法 (ICG-PDT)，HGF 细胞中 BAX 的表达显着升高。另一方面，BCL-2基因Impact表达并未受到激光照射或ICG的显着影响。

体内染色示例1
吲哚菁绿是一种无毒/低毒的荧光染料，可用于检测口服给药后多糖纳米乳液的分布
方法：用于多糖纳米乳液的分布检测
1.将吲哚菁绿溶解在多糖水溶液中，然后加入到纳米乳液中以形成络合物（吲哚菁绿在两种溶液中的最终浓度均为0.05mg/mL）
2.将9只BALB/C裸鼠分为三个实验组。小鼠在各自治疗后禁食4小时，然后置于活体成像设备（Lumina XR Series III；Perkin-Elmer，Inc）中以观察体内荧光分布。

细胞染色示例1
吲哚菁绿是一种无毒/低毒的荧光染料。它只能在静脉注射后被肝细胞吸收，然后由肝细胞分泌。当用无血清培养基培养时，肝细胞吸收吲哚菁绿，细胞质呈绿色。血清恢复后，吲哚青绿从细胞质中分泌出来
方法：用于吲哚菁绿吸收测定
1.将吲哚菁绿以1mg/mL的终浓度加入无血清肝细胞培养基中，在37℃下培养细胞1小时
2.去除无血清培养基，并用PBS洗涤细胞三次
3.用显微镜（IX71FL，OLYMPUS）对细胞进行检查和成像

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细胞染色示例2
吲哚菁绿是一种无毒/低毒的荧光染料。它可以被淋巴吸收，并在动物模型和人类受试者中使用其单光子荧光为可视化淋巴管管腔提供对比度
方法：无创2PF成像。

In Vivo Staining Example 1
Indocyanine green is a non/low-toxic fluorescent dye that can be used to detect the distribution of polysaccharide-nanoemulsion following oral administration.
Method: For polysaccharide-nanoemulsion distribution detecting.
1. Indocyanine green is dissolved in the polysaccharide aqueous solution and then added to the nanoemulsion to form an complex (The final concentration of Indocyanine green in both solutions is 0.05 mg/mL).
2. A total of 9 BALB/C nude mice are assigned to each of three experimental groups. The mice sre subjected to fasting 4 h following their respective treatment and then placed in a live imaging device (Lumina XR Series III; Perkin-Elmer, Inc) to observe fluorescence distribution in vivo.

Absorption, Distribution and Excretion
FOLLOWING IV INJECTION, INDOCYANINE GREEN IS RAPIDLY BOUND TO PLASMA PROTEIN, QUICKLY REMOVED FROM CIRCULATION BY LIVER, & EXCRETED IN BILE IN UNCONJUGATED FORM.
WITH INDOCYANINE GREEN, IT HAS BEEN SHOWN THAT RAT HAS HIGHER MAX BILIARY EXCRETION RATE (0.065 MG/KG/MIN) THAN DOES RABBIT (0.05 MG/KG/MIN) OR DOG (0.027 MG/KG/MIN).
T/2 INCR WITH INCR DOSAGE. ... CONSIDERABLE DIFFERENCE HAS BEEN NOTED BETWEEN NORMAL MONGRELS & PUREBRED BEAGLES IN CLEARANCE TIMES.
APPARENT HEPATIC EXTRACTION OF INDOCYANINE GREEN IN RATS WAS SURPRISINGLY LOW @ DOSES ABOVE 1 MG/KG. CHANGES IN HEPATIC BLOOD FLOW WOULD NOT ALTER CLEARANCE.
For more Absorption, Distribution and Excretion (Complete) data for INDOCYANINE GREEN (10 total), please visit the HSDB record page.

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Metabolism / Metabolites
...INDOCYANINE GREEN...APPEARS IN BILE UNCHANGED

Effects During Pregnancy and Lactation
◉ Summary of Use during Lactation
Data from one patient indicates that a low subcutaneous dose of indocyanine green is not detectable in breastmilk. No data are available on the user of larger intravenous doses. Until more data become available, indocyanine green should be used with caution during breastfeeding, especially while nursing a newborn or preterm infant.
◉ Effects in Breastfed Infants
Relevant published information was not found as of the revision date.
◉ Effects on Lactation and Breastmilk
Relevant published information was not found as of the revision date.

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Interactions
INCR IN HEPATIC EXCRETORY FUNCTION HAS BEEN OBSERVED AFTER PRETREATMENT WITH SOME DRUGS. FOR EXAMPLE, PHENOBARBITAL HAS BEEN DEMONSTRATED TO INCR PLASMA DISAPPEARANCE & BILIARY EXCRETION OF...INDOCYANINE GREEN...
SUPPRESSION OF BILIARY SECRETION OF INDOCYANINE GREEN...BY PRETREATMENT WITH SKF 525-A WAS SHOWN TO BE DUE TO TEMP EFFECT RATHER THAN TO EFFECT ON HEPATIC ENDOPLASMIC RETICULUM.
INDOCYANINE GREEN DECR INITIAL RATE OF UPTAKE OF OUABAIN IN RAT LIVER CELLS BY MORE THAN 50%. DATA INDICATE THAT CMPD MAY ENTER LIVER CELLS BY PROCESS SIMILAR TO ACTIVE CARRIER-MEDIATED TRANSPORT.
IN MALE RATS INJECTED IV WITH 1.0 MG/KG MERCURY, INDOCYANINE GREEN INHIBITED BILIARY EXCRETION OF METHYLMERCURY.
For more Interactions (Complete) data for INDOCYANINE GREEN (13 total), please visit the HSDB record page.

[1]. Effect of photodynamic therapy based on indocyanine green on expression of apoptosis-related genes in human gingival fibroblast cells. Photodiagnosis Photodyn Ther. 2017 Apr 21.

Indocyanine green is a cyanine dye, a benzoindole and a 1,1-diunsubstituted alkanesulfonate.
Indocyanine Green is a nontoxic, fluorescent, tricarbocyanine dye with a peak spectral absorption at 790 nm, with application in determining cardiac output, hepatic function, liver blood flow, as well as ophthalmic angiography. Upon administration, indocyanine green (ICG) rapidly binds to its principle carrier, plasma protein, and is thereby confined to the vascular space. This agent, with a half-life of 150 to 180 seconds, is removed exclusively by the liver from circulation to bile juice. Furthermore, due to poor uptake, ICG is not suitable for angiography or functional output analysis of kidney, lung, cerebro-spinal, or peripheral tissues.
A tricarbocyanine dye that is used diagnostically in liver function tests and to determine blood volume and cardiac output.
See also: indocyanine green acid form (has active moiety).

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Therapeutic Uses
Dyes
TO DETERMINE CARDIAC OUTPUT, HEPATIC FUNCTION, & LIVER BLOOD FLOW. FOR HEPATIC FUNCTION STUDIES, CALCULATED AMT OF DIAGNOSTIC AGENT IS INJECTED INTO ARM VEIN. 20 MIN AFTER INJECTION, 6 ML OF VENOUS BLOOD IS WITHDRAWN FROM OPPOSITE ARM. AFTER COAGULATION & CENTRIFUGATION, CLEAR SERUM IS READ IN PHOTOMETER @ 800-810 NM.
DYE RETENTION OF LESS THAN 4% IS FOUND IN HEALTHY SUBJECTS. ... FAILURE TO REMOVE DYE, AS INDICATED BY SERUM LEVELS IN EXCESS OF 4%, IS INDICATIVE OF IMPAIRED HEPATIC FUNCTION.
USUAL, IV, BLOOD VOL DETERMINATION, 5 MG IN 1 ML; HEPATIC FUNCTION DETERMINATION, 0-5 MG/KG.
DYE IS STABLE IN PLASMA & WHOLE BLOOD PERMITTING LATER ANALYSIS. USERS VARY BOTH CONCN & DOSAGE. IN MAN TOTAL DOSAGE HAS NORMALLY BEEN BELOW 2 MG/KG.

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Drug Warnings
...CONTAINS SMALL AMT OF /5%/ SODIUM IODIDE; THUS, IT SHOULD BE USED WITH CAUTION IN PT ALLERGIC TO IODIDES & RADIOACTIVE IODINE UPTAKE STUDIES SHOULD NOT BE PERFORMED FOR AT LEAST 1 WK FOLLOWING ITS USE. SINCE PROBENECID HAS BEEN SHOWN IN DOGS TO AFFECT HEPATIC UPTAKE, THIS POSSIBILITY SHOULD BE KEPT IN MIND.
SAFE USE OF THIS DRUG IN PREGNANCY HAS NOT BEEN ESTABLISHED.
USE FRESH SOLN ONLY AS RECOMMENDED.

C43H47N2NAO6S2

774.9629

774.277

C, 66.64; H, 6.11; N, 3.61; Na, 2.97; O, 12.39; S, 8.27

3599-32-4

5282412

Light green to dark green solid powder

157ºC

235 °C

10.555

137.41

0

7

12

54

1520

0

S(C([H])([H])C([H])([H])C([H])([H])C([H])([H])[N+]1C2C([H])=C([H])C3=C([H])C([H])=C([H])C([H])=C3C=2C(C([H])([H])[H])(C([H])([H])[H])C=1/C(/[H])=C(/[H])\C(\[H])=C(/[H])\C(\[H])=C(/[H])\C(\[H])=C1/C(C([H])([H])[H])(C([H])([H])[H])C2C3=C([H])C([H])=C([H])C([H])=C3C([H])=C([H])C=2N/1C([H])([H])C([H])([H])C([H])([H])C([H])([H])S(=O)(=O)[O-])(=O)(=O)[O-].[Na+]

MOFVSTNWEDAEEK-UHFFFAOYSA-M

InChI=1S/C43H48N2O6S2.Na/c1-42(2)38(44(28-14-16-30-52(46,47)48)36-26-24-32-18-10-12-20-34(32)40(36)42)22-8-6-5-7-9-23-39-43(3,4)41-35-21-13-11-19-33(35)25-27-37(41)45(39)29-15-17-31-53(49,50)51/h5-13,18-27H,14-17,28-31H2,1-4H3,(H-,46,47,48,49,50,51)/q+1/p-1

sodium 4-(2-((1E,3E,5E,7E)-7-(1,1-dimethyl-3-(4-sulfonatobutyl)-1,3-dihydro-2H-benzo[e]indol-2-ylidene)hepta-1,3,5-trien-1-yl)-1,1-dimethyl-1H-benzo[e]indol-3-ium-3-yl)butane-1-sulfonate

Cardio-Green Fox IR-125 IR 125 IR125

2934.99.9001

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

注意： 请将本产品存放在密封且受保护的环境中，避免吸湿/受潮和光照。

Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

DMSO : ~83.33 mg/mL (~107.53 mM)
H2O : ~5 mg/mL (~6.45 mM)

配方 1 中的溶解度: ≥ 2.5 mg/mL (3.23 mM) (饱和度未知) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (这些助溶剂从左到右依次添加，逐一添加), 澄清溶液。
例如，若需制备1 mL的工作液，可将100 μL 25.0 mg/mL澄清DMSO储备液加入到400 μL PEG300中，混匀；然后向上述溶液中加入50 μL Tween-80，混匀；加入450 μL生理盐水定容至1 mL。
*生理盐水的制备：将 0.9 g 氯化钠溶解在 100 mL ddH₂O中，得到澄清溶液。

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配方 2 中的溶解度: 2.5 mg/mL (3.23 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (这些助溶剂从左到右依次添加，逐一添加), 悬浊液; 超声助溶。
例如，若需制备1 mL的工作液，可将 100 μL 25.0 mg/mL澄清DMSO储备液加入900 μL 20% SBE-β-CD生理盐水溶液中，混匀。
*20% SBE-β-CD 生理盐水溶液的制备（4°C，1 周）：将 2 g SBE-β-CD 溶解于 10 mL 生理盐水中，得到澄清溶液。

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请根据您的实验动物和给药方式选择适当的溶解配方/方案：
1、请先配制澄清的储备液(如：用DMSO配置50 或 100 mg/mL母液(储备液));
2、取适量母液，按从左到右的顺序依次添加助溶剂，澄清后再加入下一助溶剂。以 下列配方为例说明 (注意此配方只用于说明，并不一定代表此产品 的实际溶解配方):
10% DMSO → 40% PEG300 → 5% Tween-80 → 45% ddH2O (或 saline);
假设最终工作液的体积为 1 mL, 浓度为5 mg/mL: 取 100 μL 50 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀/澄清；向上述体系中加入50 μL Tween-80，混合均匀/澄清；然后继续加入450 μL ddH2O (或 saline)定容至 1 mL；
3、溶剂前显示的百分比是指该溶剂在最终溶液/工作液中的体积所占比例;
4、 如产品在配制过程中出现沉淀/析出，可通过加热(≤50℃)或超声的方式助溶;
5、为保证最佳实验结果，工作液请现配现用！
6、如不确定怎么将母液配置成体内动物实验的工作液，请查看说明书或联系我们;
7、 以上所有助溶剂都可在 Invivochem.cn网站购买。