Oxaloacetic acid   中文名称: 草酰乙酸

Human Endogenous Metabolite

草酰乙酸(10mg/kg，每天1次，连续3天)能明显减轻百草枯(50mg/kg，一次)引起的急性肺损伤，提高百草枯中毒小鼠的存活率[3]。

[1]. F. L. Breusch. The fate of oxaloacetic acid in different organs. Biochem J. 1939 Nov; 33(11): 1757-1770.

[2]. Oxaloacetic acid mediates ADP-dependent inhibition of mitochondrial complex II-driven respiration. J Biol Chem. 2018 Dec 21;293(51):19932-19941.

[3]. Oxaloacetate acid ameliorates paraquat-induced acute lung injury by alleviating oxidative stress and mitochondrial dysfunction. Front Pharmacol. 2022 Oct 13;13:1029775.

Oxaloacetic acid is an oxodicarboxylic acid that is succinic acid bearing a single oxo group. It has a role as a metabolite and a geroprotector. It is an oxo dicarboxylic acid and a C4-dicarboxylic acid. It is functionally related to a succinic acid. It is a conjugate acid of an oxaloacetate(2-).
Oxalacetic acid is a metabolite found in or produced by Escherichia coli (strain K12, MG1655).
Oxalacetic acid has been reported in Drosophila melanogaster, Medicago sativa, and other organisms with data available.
Anhydrous Enol-oxaloacetate is the anhydrous form of enol-oxaloacetate, a small molecule blood glutamate scavenger, that can be used to lower glutamate plasma levels, and has potential neuroprotective activity. Upon administration, enol-oxaloacetate targets and binds to glutamate in the bloodstream. This lowers glutamate plasma levels and lowers the free glutamate available to be picked up by cells, such as tumor brain cells, thereby preventing glutamate metabolism and glutamate-mediated signaling. This prevents the proliferation of rapidly growing cells, such as brain tumor cells. And by lowering glutamate plasma levels, a molecular imbalance is formed and glutamate is excreted across the blood-brain barrier, resulting in lower free brain glutamate. This may help protect the brain from excitotoxicity in conditions where there is a surge of glutamate production, such as traumatic brain injury, thereby protecting neuronal cells. Glutamate, a non-essential amino acid and the major excitatory neurotransmitter in the central nervous system (CNS), provides energy and generates building blocks for the production of macromolecules, which are needed for cellular growth and survival.
Oxalacetic acid is a metabolite found in or produced by Saccharomyces cerevisiae.
A dicarboxylic acid ketone that is an important metabolic intermediate of the CITRIC ACID CYCLE. It can be converted to ASPARTIC ACID by ASPARTATE TRANSAMINASE.

C4H4O5

132.07

132.005

328-42-7

970

Solid powder

1.6±0.1 g/cm3

341.9±25.0 °C at 760 mmHg

161 °C (dec.)(lit.)

174.8±19.7 °C

0.0±1.6 mmHg at 25°C

1.498

-1.6

91.67

2

5

3

9

158

0

C(C(=O)C(=O)O)C(=O)O

KHPXUQMNIQBQEV-UHFFFAOYSA-N

InChI=1S/C4H4O5/c5-2(4(8)9)1-3(6)7/h1H2,(H,6,7)(H,8,9)

2-oxobutanedioic acid

Oxaloacetic acid; 2-Oxosuccinic acid

2934.99.9001

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

H2O : ≥ 100 mg/mL (757.17 mM) DMSO : ≥ 100 mg/mL (757.17 mM)

配方 1 中的溶解度: 100 mg/mL (757.17 mM)) in PBS (这些助溶剂从左到右依次添加，逐一添加),澄清溶液; 超声助溶。 请根据您的实验动物和给药方式选择适当的溶解配方/方案：
1、请先配制澄清的储备液(如：用DMSO配置50 或 100 mg/mL母液(储备液));
2、取适量母液，按从左到右的顺序依次添加助溶剂，澄清后再加入下一助溶剂。以 下列配方为例说明 (注意此配方只用于说明，并不一定代表此产品 的实际溶解配方):
10% DMSO → 40% PEG300 → 5% Tween-80 → 45% ddH2O (或 saline);
假设最终工作液的体积为 1 mL, 浓度为5 mg/mL: 取 100 μL 50 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀/澄清；向上述体系中加入50 μL Tween-80，混合均匀/澄清；然后继续加入450 μL ddH2O (或 saline)定容至 1 mL；
3、溶剂前显示的百分比是指该溶剂在最终溶液/工作液中的体积所占比例;
4、 如产品在配制过程中出现沉淀/析出，可通过加热(≤50℃)或超声的方式助溶;
5、为保证最佳实验结果，工作液请现配现用！
6、如不确定怎么将母液配置成体内动物实验的工作液，请查看说明书或联系我们;
7、 以上所有助溶剂都可在 Invivochem.cn网站购买。