一个额外的甲基使盐酸去甲肾上腺素成为一种去甲肾上腺素代谢物[2]。

1: Marušić R, Dragila Ž, Levaković A, Lekić I, Mihaljević D, Debeljak Ž, Bačun T. The most common indications for measuring metanephrine and normetanephrine in plasma - a rational approach. Med Glas (Zenica). 2023 Aug 1;20(2). doi: 10.17392/1588-23. Epub ahead of print. PMID: 37300464. 2: Prego MT, Dias MJ, Ferreira RL, Gonçalves S, Dias Domingues T, Junius G, Van den Steen E, Galac S, Leal RO. Plasma and urinary metanephrine and normetanephrine concentrations using liquid chromatography with tandem mass spectrometry in healthy cats and in a cat with pheochromocytoma. J Vet Intern Med. 2023 May-Jun;37(3):910-914. doi: 10.1111/jvim.16648. Epub 2023 Apr 13. PMID: 37057306; PMCID: PMC10229322. 3: Yu Y, Chen C, Han W, Zhang Y, Zhang Z, Yang Y. Metanephrine and normetanephrine associated with subclinical myocardial injuries in pheochromocytoma and paraganglioma. Front Oncol. 2022 Sep 27;12:1024342. doi: 10.3389/fonc.2022.1024342. PMID: 36237312; PMCID: PMC9552905. 4: Deng Y, Liu Q, Liu Z, Zhao H, Zhou W, Zhang C. Commutability Assessment of Processed Human Plasma Samples for Normetanephrine and Metanephrine Measurements Based on the Candidate Reference Measurement Procedure. Ann Lab Med. 2022 Sep 1;42(5):575-584. doi: 10.3343/alm.2022.42.5.575. PMID: 35470275; PMCID: PMC9057823. 5: Remde H, Pamporaki C, Quinkler M, Nölting S, Prejbisz A, Timmers HJLM, Masjkur J, Fuss CT, Fassnacht M, Eisenhofer G, Deutschbein T. Improved Diagnostic Accuracy of Clonidine Suppression Testing Using an Age-Related Cutoff for Plasma Normetanephrine. Hypertension. 2022 Jun;79(6):1257-1264. doi: 10.1161/HYPERTENSIONAHA.122.19019. Epub 2022 Apr 5. PMID: 35378989. 6: Liu W, Zhang J, Yang Y, Jin Y, Li Z, You L, Luo J, Su X. Application of Metanephrine and Normetanephrine in Evaluating the Selectivity of Adrenal Vein Sampling. Horm Metab Res. 2022 Mar;54(3):162-167. doi: 10.1055/a-1756-4937. Epub 2022 Mar 11. PMID: 35276741. 7: Höglund K, Palmqvist H, Ringmark S, Svensson A. Quantification of normetanephrine in canine urine using ELISA: evaluation of factors affecting results. J Vet Diagn Invest. 2022 Jan;34(1):28-35. doi: 10.1177/10406387211052984. Epub 2021 Oct 26. PMID: 34697963; PMCID: PMC8689017. 8: Kline GA, Boyd J, Hyrcza M, Pacaud D, Pasieka JL, Dong Y, Sadrzadeh HSM, Leung AAC. Updated reference intervals for urine normetanephrine have no effect on test sensitivity but fewer false positives. Clin Biochem. 2022 Jan;99:17-19. doi: 10.1016/j.clinbiochem.2021.09.013. Epub 2021 Oct 6. PMID: 34626610. 9: Peitzsch M, Novos T, Kaden D, Kurlbaum M, van Herwaarden AE, Müller D, Adaway J, Grouzmann E, McWhinney B, Hoad K, Woollard G, Kema I, Boot C, Fassnacht M, Sweep F, Loh TP, Horvath AR, Eisenhofer G. Harmonization of LC-MS/MS Measurements of Plasma Free Normetanephrine, Metanephrine, and 3-Methoxytyramine. Clin Chem. 2021 Aug 5;67(8):1098-1112. doi: 10.1093/clinchem/hvab060. PMID: 33993248. 10: Mizuno K, Sakaue H, Kohsaka K, Takeda H, Hayashi N, Sato T. An increase in normetanephrine in hair follicles of acne lesions through the sympatho-adrenal medullary system in acne patients with anxiety. J Dermatol. 2021 Aug;48(8):1281-1285. doi: 10.1111/1346-8138.15935. Epub 2021 May 7. PMID: 33963607. 11: Kline GA, Boyd J, Leung AA, Tang A, Sadrzadeh HM. Moderate renal impairment does not preclude the accuracy of 24-hour urine normetanephrine measurements for suspected pheochromoctyoma. Clin Endocrinol (Oxf). 2020 Jun;92(6):518-524. doi: 10.1111/cen.14180. Epub 2020 Mar 18. PMID: 32133686. 12: Murabayashi M, Daimon M, Murakami H, Fujita T, Sato E, Tanabe J, Matsuhashi Y, Takayasu S, Yanagimachi M, Terui K, Kageyama K, Tokuda I, Sawada K, Ihara K. Association between higher urinary normetanephrine and insulin resistance in a Japanese population. PLoS One. 2020 Feb 13;15(2):e0228787. doi: 10.1371/journal.pone.0228787. PMID: 32053635; PMCID: PMC7018048. 13: Peitzsch M, Butch ER, Lovorn E, Mangelis A, Furman WL, Santana VM, Hero B, Berthold F, Shulkin BL, Huebner A, Eisenhofer G. Biochemical testing for neuroblastoma using plasma free 3-O-methyldopa, 3-methoxytyramine, and normetanephrine. Pediatr Blood Cancer. 2020 Feb;67(2):e28081. doi: 10.1002/pbc.28081. Epub 2019 Nov 14. PMID: 31724812. 14: Boyd J, Leung AA, Sadrzadeh HS, Pamporaki C, Pacak K, Deutschbein T, Fliedner S, Kline GA. A high rate of modestly elevated plasma normetanephrine in a population referred for suspected PPGL when measured in a seated position. Eur J Endocrinol. 2019 Sep;181(3):301-309. doi: 10.1530/EJE-19-0176. PMID: 31370003; PMCID: PMC7446766. 15: Peitzsch M, Mangelis A, Eisenhofer G, Huebner A. Age-specific pediatric reference intervals for plasma free normetanephrine, metanephrine, 3-methoxytyramine and 3-O-methyldopa: Particular importance for early infancy. Clin Chim Acta. 2019 Jul;494:100-105. doi: 10.1016/j.cca.2019.03.1620. Epub 2019 Mar 20. PMID: 30904545. 16: Godoy-Reyes TM, Costero AM, Gaviña P, Martínez-Máñez R, Sancenón F. Colorimetric detection of normetanephrine, a pheochromocytoma biomarker, using bifunctionalised gold nanoparticles. Anal Chim Acta. 2019 May 16;1056:146-152. doi: 10.1016/j.aca.2019.01.003. Epub 2019 Jan 8. PMID: 30797455. 17: Eisenhofer G, Peitzsch M, Kaden D, Langton K, Mangelis A, Pamporaki C, Masjkur J, Geroula A, Kurlbaum M, Deutschbein T, Beuschlein F, Prejbisz A, Bornstein SR, Lenders JWM. Reference intervals for LC-MS/MS measurements of plasma free, urinary free and urinary acid-hydrolyzed deconjugated normetanephrine, metanephrine and methoxytyramine. Clin Chim Acta. 2019 Mar;490:46-54. doi: 10.1016/j.cca.2018.12.019. Epub 2018 Dec 17. PMID: 30571948. 18: van den Ouweland JM, Mol M, Tax L, Beijers T, van Daal H. α-Methyldopa Interference in Urinary Normetanephrine Measurement by LC-MS/MS? J Appl Lab Med. 2016 Nov 1;1(3):321-324. doi: 10.1373/jalm.2016.020925. PMID: 33626849. 19: Bílek R, Zelinka T, Vlček P, Dušková J, Michalský D, Novák K, Bešťák J, Widimský J Jr. Deconjugated urinary metanephrine, normetanephrine and 3-methoxytyramine in laboratory diagnosis of pheochromocytoma and paraganglioma. Physiol Res. 2015;64(Suppl 2):S313-22. doi: 10.33549/physiolres.933109. PMID: 26680494. 20: Gabler J, Wang S. Quantification of Metanephrine and Normetanephrine in Urine Using Liquid Chromatography-Tandem Mass Spectrometry. Methods Mol Biol. 2016;1378:149-57. doi: 10.1007/978-1-4939-3182-8_17. PMID: 26602127.

C9H14CLNO3

219.665

219.066

C, 49.21; H, 6.42; Cl, 16.14; N, 6.38; O, 21.85

1011-74-1

Normetanephrine-d3 hydrochloride;1085333-97-6

102542

White to light yellow solid powder

393.8ºC at 760mmHg

204ºC (dec.)(lit.)

192ºC

1.13E-07mmHg at 25°C

1.895

75.71

4

4

3

14

154

0

VKFPRGQZWKTEON-UHFFFAOYSA-N

InChI=1S/C9H13NO3.ClH/c1-13-9-4-6(8(12)5-10)2-3-7(9)11;/h2-4,8,11-12H,5,10H2,1H3;1H

4-(2-amino-1-hydroxyethyl)-2-methoxyphenol hydrochloride

Normetanephrine HCl; Normetanephrine hydrochloride; DL-α-(Aminomethyl)-4-hydroxy-3-methoxybenzenemethanol hydrochloride;

2934.99.9001

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

注意： 请将本产品存放在密封且受保护的环境中（例如氮气保护），避免吸湿/受潮和光照。

Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

H2O : ~100 mg/mL (~455.23 mM)
DMSO : ~20.83 mg/mL (~94.82 mM)

配方 1 中的溶解度: ≥ 2.08 mg/mL (9.47 mM) (饱和度未知) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (这些助溶剂从左到右依次添加，逐一添加), 澄清溶液。
例如，若需制备1 mL的工作液，可将100 μL 20.8 mg/mL澄清DMSO储备液加入400 μL PEG300中，混匀；然后向上述溶液中加入50 μL Tween-80，混匀；加入450 μL生理盐水定容至1 mL。
*生理盐水的制备：将 0.9 g 氯化钠溶解在 100 mL ddH₂O中，得到澄清溶液。

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配方 2 中的溶解度: ≥ 2.08 mg/mL (9.47 mM) (饱和度未知) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (这些助溶剂从左到右依次添加，逐一添加), 澄清溶液。
例如，若需制备1 mL的工作液，可将 100 μL 20.8 mg/mL澄清DMSO储备液加入900 μL 20% SBE-β-CD生理盐水溶液中，混匀。
*20% SBE-β-CD 生理盐水溶液的制备（4°C，1 周）：将 2 g SBE-β-CD 溶解于 10 mL 生理盐水中，得到澄清溶液。

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配方 3 中的溶解度: ≥ 2.08 mg/mL (9.47 mM) (饱和度未知) in 10% DMSO + 90% Corn Oil (这些助溶剂从左到右依次添加，逐一添加), 澄清溶液。
例如，若需制备1 mL的工作液，可将 100 μL 20.8 mg/mL 澄清 DMSO 储备液加入到 900 μL 玉米油中并混合均匀。

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请根据您的实验动物和给药方式选择适当的溶解配方/方案：
1、请先配制澄清的储备液(如：用DMSO配置50 或 100 mg/mL母液(储备液));
2、取适量母液，按从左到右的顺序依次添加助溶剂，澄清后再加入下一助溶剂。以 下列配方为例说明 (注意此配方只用于说明，并不一定代表此产品 的实际溶解配方):
10% DMSO → 40% PEG300 → 5% Tween-80 → 45% ddH2O (或 saline);
假设最终工作液的体积为 1 mL, 浓度为5 mg/mL: 取 100 μL 50 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀/澄清；向上述体系中加入50 μL Tween-80，混合均匀/澄清；然后继续加入450 μL ddH2O (或 saline)定容至 1 mL；
3、溶剂前显示的百分比是指该溶剂在最终溶液/工作液中的体积所占比例;
4、 如产品在配制过程中出现沉淀/析出，可通过加热(≤50℃)或超声的方式助溶;
5、为保证最佳实验结果，工作液请现配现用！
6、如不确定怎么将母液配置成体内动物实验的工作液，请查看说明书或联系我们;
7、 以上所有助溶剂都可在 Invivochem.cn网站购买。