Natural flavone; 5-HT, NE, DA and GABA

结果：500 mg kg-1剂量的PSL水提取物（基于以往经验），以及不同浓度的上述四种功能成分（189.86µg kg-1芳樟醇、114.75 mg kg-1 5-羟基-4'，7-二甲氧基黄烷酮、32.4 mg kg-1 2'-O-甲基异甘草酸和44.44 mg kg-1阿魏酸），通过影响小鼠的神经递质水平，具有镇静催眠作用。 结论：数据表明，这四种成分是PSL水提取物镇静催眠和抗焦虑作用的关键功能因素，这些作用是通过神经递质水平和途径的变化发生的。

[1]. Sedative-hypnotic and Anxiolytic Effects and the Mechanism of Action of Aqueous Extracts of Peanut Stems and Leaves in Mice. J Sci Food Agric. 2018 Oct;98(13):4885-4894.

2'-O-methylisoliquiritigenin is a member of the class of chalcones that is isoliquiritigenin in which one of the hydroxy groups at position 2' is replaced by a methoxy group. It has a role as a metabolite. It is a member of chalcones, a monomethoxybenzene and a member of phenols. It is functionally related to an isoliquiritigenin.
2'-O-Methylisoliquiritigenin has been reported in Dracaena cochinchinensis, Dracaena cambodiana, and other organisms with data available.

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Peanut stems and leaves (PSL) have traditionally been used as both a special food and a herbal medicine in Asia. The sedative-hypnotic and anxiolytic effects of PSL have been recorded in classical traditional Chinese literature, and more recently by many other researchers. In a previous study, four sleep-related ingredients (linalool, 5-hydroxy-4',7-dimethoxyflavanone, 2'-O-methylisoliquiritigenin and ferulic acid), among which 5-hydroxy-4',7-dimethoxyflavanone and 2'-O-methylisoliquiritigenin were newly found in Arachis species, were screened by ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC/QTOF-MS). In the current study, quantitative examination of the above four ingredients was conducted. Serious fundamental functional studies were done in mice, including locomotor activity, direct sleep tests, pentobarbital-induced sleeping time tests, subthreshold dose of pentobarbital tests and barbital sodium sleep incubation period tests, to determine the material base for the sedative-hypnotic and anxiolytic effects of aqueous extracts of PSL. Furthermore, neurotransmitter levels in three brain regions (cerebrum, cerebellum and brain stem) were determined using UHPLC coupled with triple-quadrupole mass spectrometry (UHPLC/QQQ-MS) in order to elucidate the exact mechanism of action.[1]

C16H14O4

270.28

270.089

51828-10-5

3-Deoxysappanchalcone;112408-67-0

5319688

Light yellow to yellow solid powder

1.3±0.1 g/cm3

527.1±50.0 °C at 760 mmHg

210 - 212 °C

200.5±23.6 °C

0.0±1.4 mmHg at 25°C

1.658

2.96

66.76

2

4

4

20

344

0

COC1=C(C=CC(=C1)O)C(=O)/C=C/C2=CC=C(C=C2)O

PACBGANPVNHGNP-RUDMXATFSA-N

InChI=1S/C16H14O4/c1-20-16-10-13(18)7-8-14(16)15(19)9-4-11-2-5-12(17)6-3-11/h2-10,17-18H,1H3/b9-4+

(E)-1-(4-hydroxy-2-methoxyphenyl)-3-(4-hydroxyphenyl)prop-2-en-1-one

3-Deoxysappanchalcone; 2'-O-Methylisoliquiritigenin; 112408-67-0; 4,4'-dihydroxy-2'-methoxychalcone; 2'-Methoxyisoliquiritigenin; CHEBI:519567; LHE9JFQ1U8; ...; 51828-10-5;

2934.99.9001

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

注意： 本产品在运输和储存过程中需避光。

Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

DMSO: 100 mg/mL (369.99 mM)

配方 1 中的溶解度: ≥ 2.5 mg/mL (9.25 mM) (饱和度未知) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (这些助溶剂从左到右依次添加，逐一添加), 澄清溶液。
例如，若需制备1 mL的工作液，可将100 μL 25.0 mg/mL澄清DMSO储备液加入到400 μL PEG300中，混匀；然后向上述溶液中加入50 μL Tween-80+，混匀；加入450 μL生理盐水定容至1 mL。
*生理盐水的制备：将 0.9 g 氯化钠溶解在 100 mL ddH₂O中，得到澄清溶液。

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请根据您的实验动物和给药方式选择适当的溶解配方/方案：
1、请先配制澄清的储备液(如：用DMSO配置50 或 100 mg/mL母液(储备液));
2、取适量母液，按从左到右的顺序依次添加助溶剂，澄清后再加入下一助溶剂。以 下列配方为例说明 (注意此配方只用于说明，并不一定代表此产品 的实际溶解配方):
10% DMSO → 40% PEG300 → 5% Tween-80 → 45% ddH2O (或 saline);
假设最终工作液的体积为 1 mL, 浓度为5 mg/mL: 取 100 μL 50 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀/澄清；向上述体系中加入50 μL Tween-80，混合均匀/澄清；然后继续加入450 μL ddH2O (或 saline)定容至 1 mL；
3、溶剂前显示的百分比是指该溶剂在最终溶液/工作液中的体积所占比例;
4、 如产品在配制过程中出现沉淀/析出，可通过加热(≤50℃)或超声的方式助溶;
5、为保证最佳实验结果，工作液请现配现用！
6、如不确定怎么将母液配置成体内动物实验的工作液，请查看说明书或联系我们;
7、 以上所有助溶剂都可在 Invivochem.cn网站购买。