Hydroxyproline   中文名称: L-羟基脯氨酸

L-羟基脯氨酸，又称Trans-4-羟基-L-脯氨酸或Trans-Hyp，广泛应用于食品、化妆品、生化、医疗等行业。此外，还发现一些次级代谢产物，如放线菌素和棘白菌素，包含L-羟脯氨酸。由于L-羟脯氨酸富含胶原蛋白，工业上大多通过酸水解哺乳动物胶原蛋白来生产[1]。

[1]. Biosynthesis of trans-4-hydroxyproline by recombinant strains of Corynebacterium glutamicum and Escherichia coli. BMC Biotechnol. 2014 May 19;14:44.

Trans-4-hydroxy-L-proline is an optically active form of 4-hydroxyproline, exhibiting the L-trans configuration. It is found in humans, plants, and mice, and is a zwitterion tautomer of trans-4-hydroxy-L-proline. Hydroxyproline is a neutral heterocyclic protein amino acid found in collagen and is therefore commonly found in many gelatin products. Hydroxyproline is primarily used as a diagnostic marker for bone turnover and liver fibrosis. In terms of treatment, hydroxyproline is being investigated as an experimental drug, but it has been approved in France for the treatment of small, superficial wounds, in a compound topical gel product called Cicactive. Hydroxyproline is a metabolite found in or produced by Escherichia coli (K12 strain, MG1655 strain). 4-Hydroxy-L-proline is a metabolite found in or produced by Escherichia coli (K12 strain, MG1655 strain). Hydroxyproline has been reported in Daphnia pulex, Glycine max, and other organisms with relevant data. Hydroxyproline is a non-essential amino acid derivative formed during post-translational modification of proteins by the hydroxylation of the amino acid proline by prolyl hydroxylase, an enzyme that requires vitamin C as a cofactor. Hydroxyproline is a major component of collagen and plays a crucial role in maintaining the stability of the collagen triple helix structure. Hydroxyproline can be used as an indicator of collagen content. Elevated levels of hydroxyproline in urine and/or serum are often associated with connective tissue degradation. Vitamin C deficiency reduces the conversion of proline to hydroxyproline, leading to decreased collagen stability. 4-Hydroxyproline (or hydroxyproline or Hyp) is a major component of collagen. Hydroxyproline is generated by the hydroxylation of the amino acid proline, and is therefore a post-translational modified amino acid. Hydroxyproline and proline play crucial roles in maintaining collagen stability. In particular, they can cause significant distortion of the collagen helix. Hydroxyproline is rarely found in other proteins besides collagen. The only protein in mammals containing hydroxyproline is elastin. Therefore, hydroxyproline content has been used as an indicator of collagen and/or gelatin content in tissue or biological samples. Serum and urine hydroxyproline levels are elevated in patients with Paget's disease. Hydroxyproline (Hyp) content in bodily fluids has been used as an indicator of collagen catabolism, particularly related to bone resorption or tissue degradation. Serum hydroxyproline levels are significantly higher in bedridden and elderly individuals than in normally active individuals. Elevated urinary hydroxyproline levels also suggest muscle damage. Increased reactive oxygen species (ROS) are known to accelerate collagen degradation. Hydroxyproline levels are also elevated under conditions of depression and stress (A3486, A3487, A3488, A3489). See also: Hydroxyproline; Octyl methoxycinnamate (ingredient); Hydroxyproline; Nicotinamide (ingredient)... See more...

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Drug Indications
In France, this product is used as a compound preparation for the treatment of small, superficial wounds.

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Pharmacodynamics
Hydroxyproline, along with proline and glycine, are major components of collagen. Collagen is one of the main components of connective tissues such as skin, bones, and cartilage. Therefore, when these tissues are damaged, hydroxyproline is essential for repairing damaged areas.

C5H9NO3

131.13

131.058

51-35-4

25249-07-4

5810

White to off-white solid powder

1.4±0.1 g/cm3

355.2±42.0 °C at 760 mmHg

273 °C (dec.)(lit.)

168.6±27.9 °C

0.0±1.8 mmHg at 25°C

1.540

-1.84

69.56

3

4

1

9

125

2

O([H])C1([H])C([H])([H])N([H])[C@]([H])(C(=O)O[H])C1([H])[H]

PMMYEEVYMWASQN-DMTCNVIQSA-N

InChI=1S/C5H9NO3/c7-3-1-4(5(8)9)6-2-3/h3-4,6-7H,1-2H2,(H,8,9)/t3-,4+/m1/s1

(2S,4R)-4-hydroxypyrrolidine-2-carboxylic acid

NSC 46704; Oxaceprol; Hydroxyproline

2934.99.9001

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

H2O : ~50 mg/mL (~381.30 mM)

配方 1 中的溶解度: 100 mg/mL (762.60 mM) in PBS (这些助溶剂从左到右依次添加，逐一添加), 澄清溶液; 超声助溶。

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请根据您的实验动物和给药方式选择适当的溶解配方/方案：
1、请先配制澄清的储备液(如：用DMSO配置50 或 100 mg/mL母液(储备液));
2、取适量母液，按从左到右的顺序依次添加助溶剂，澄清后再加入下一助溶剂。以 下列配方为例说明 (注意此配方只用于说明，并不一定代表此产品 的实际溶解配方):
10% DMSO → 40% PEG300 → 5% Tween-80 → 45% ddH2O (或 saline);
假设最终工作液的体积为 1 mL, 浓度为5 mg/mL: 取 100 μL 50 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀/澄清；向上述体系中加入50 μL Tween-80，混合均匀/澄清；然后继续加入450 μL ddH2O (或 saline)定容至 1 mL；
3、溶剂前显示的百分比是指该溶剂在最终溶液/工作液中的体积所占比例;
4、 如产品在配制过程中出现沉淀/析出，可通过加热(≤50℃)或超声的方式助溶;
5、为保证最佳实验结果，工作液请现配现用！
6、如不确定怎么将母液配置成体内动物实验的工作液，请查看说明书或联系我们;
7、 以上所有助溶剂都可在 Invivochem.cn网站购买。