c-Kit (IC50 ~100 nM); Bcr-Abl (IC50 ~100 nM); PDGFR (IC50 ~100 nM)
Imatinib Mesylate (STI571; Gleevec; Glivec) potently inhibits c-kit receptor tyrosine kinase with an IC₅₀ of 0.01 μM [1]
It inhibits ABL tyrosine kinase (IC₅₀ = 0.025 μM) and ARG tyrosine kinase (IC₅₀ = 0.05 μM) [3]
It also inhibits imatinib-resistant KIT gatekeeper mutant (V654A) with an IC₅₀ of 0.1 μM and PDGFRβ gatekeeper mutant (T681I) with an IC₅₀ of 0.15 μM [2]

抑制一组酪氨酸和丝氨酸/苏氨酸蛋白激酶的体外试验表明，伊马替尼可有效抑制 v-Abl 酪氨酸激酶和 PDGFR，IC50 分别为 0.6 和 0.1 μM。伊马替尼抑制野生型 c-kit 激酶活性的 SLF 依赖性激活，这些作用的 IC50 约为 0.1 μM，与抑制 PDGFR 所需的浓度相似。伊马替尼对人支气管类癌细胞系 NCI-H727 和人胰腺类癌细胞系 BON-1 表现出生长抑制活性，IC50 分别为 32.4 和 32.8 μM。最近的一项研究表明，伊马替尼有可能通过下调 hERG1 K(+) 通道在慢性粒细胞白血病中发挥抗白血病作用，hERG1 K(+) 通道在白血病细胞中高表达，对于促进白血病发生具有特殊的重要性。激酶测定：PDGF 受体从 BALB/c 3T3 细胞提取物中用针对鼠 PDGF 受体的兔抗血清在冰上免疫沉淀 2 小时。 Protein A-Sepharose 珠用于收集抗原-抗体复合物。免疫沉淀物用 TNET（50 mM Tris，pH 7.5，140 mM NaCl，5 mM EDTA，1% Triton X-100）洗涤两次，用 TNE（50 mM Tris，pH 7.5，140 mM EDTA）洗涤一次，用激酶缓冲液（20 mM Tris，pH 7.5，10 mM MgCl2）。在 4°C 下用 PDGF (50 ng/mL) 刺激 10 分钟后，将不同浓度的药物添加到反应混合物中。 PDGF受体激酶活性通过与10μCi[7-33P]-ATP和1μM ATP在4℃下孵育10分钟来测定。通过 7.5% 凝胶上的 SDS-PAGE 分离免疫复合物。细胞测定：将BON-1细胞和NCI-H727细胞一式三份接种到平底96孔板中，并分别在补充有10%胎牛血清的DMEM或RPMI 1640完全培养基中贴壁过夜；然后将培养基更换为无血清培养基（阴性对照）或含有伊马替尼连续稀释液的无血清培养基。 48小时后（对照培养物未达到汇合），通过3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四唑溴化物测定法测定代谢活跃细胞的数量，并在吸光度测定中测量吸光度。 Packard Spectra 酶标仪，540 nm。使用以下公式计算生长抑制：抑制率=（1−a/b）×100%，其中a和b分别是处理组和对照组的吸光度值。

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伊马替尼甲磺酸盐（STI571；格列卫；Glivec）剂量依赖性抑制c-kit阳性肿瘤细胞系增殖，包括HMC-1肥大细胞白血病细胞（IC₅₀=0.03μM）和GIST882胃肠道间质瘤细胞（IC₅₀=0.04μM）。浓度≥0.05μM时，可阻断c-kit磷酸化及下游PI3K/AKT信号通路[1]
在A549非小细胞肺癌细胞中，该药物（1μM）与维生素D类似物协同作用，抑制细胞增殖约70%，增强维生素D诱导的G1期细胞周期阻滞[4]
2μM浓度下抑制乳腺癌细胞（MDA-MB-231）的侵袭伪足形成，通过抑制ABL激酶介导的肌动蛋白细胞骨架重排，减少细胞侵袭约65%[6]
诱导伊马替尼敏感的KIT突变细胞凋亡，EC₅₀=0.08μM，上调切割型caspase-3和PARP的表达[2]

伊马替尼对源自新鲜人小细胞肺癌手术样本的三种异种移植肿瘤产生不同的抗肿瘤作用，对 SCLC6、SCLC61 和 SCLC108 肿瘤的生长分别有 80%、40% 和 78% 的抑制作用，对 SCLC74 肿瘤的生长没有显着抑制作用。在高脂喂养的 ApoE(-/-) 小鼠中，与高脂饮食未处理的对照组相比，在 10、20 和 40 岁灌胃给药时，伊马替尼显着减少了高脂诱导的脂质染色面积 30%、27% 和 35%。 mg/kg，分别抑制颈动脉脂质积累

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伊马替尼甲磺酸盐（STI571；格列卫；Glivec）与维生素D类似物联合使用时，显著抑制裸鼠A549异种移植瘤生长。以50mg/kg/天的剂量口服给药28天，联合维生素D组的肿瘤体积较对照组减少约80%[4]
在大鼠肺缺血再灌注损伤模型中，该药物（10mg/kg，缺血前30分钟腹腔注射）通过降低氧化应激和炎症细胞因子（TNF-α、IL-6）水平约50%，减轻肺组织损伤[5]
以40mg/kg/天的剂量口服给药30天，延长携带GIST882异种移植瘤小鼠的生存期40%[1]

使用兔抗血清对 BALB/c 3T3 细胞提取物中的 PDGF 受体进行免疫沉淀，然后将其置于冰上两小时。使用 Protein A-Sepharose 珠收集抗原-抗体复合物。 TNET（50 mM Tris，pH 7.5、140 mM NaCl、5 mM EDTA、1% Triton X-100）、TNE（50 mM Tris，pH 7.5、140 mM EDTA）和激酶缓冲液（20 mM Tris，pH 7.5， 10 mM MgCl2）是用于洗涤免疫沉淀两次的三种溶液。 PDGF (50 ng/mL) 在 4°C 刺激 10 分钟后，将各种药物浓度添加到反应混合物中。

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将重组c-kit受体酪氨酸激酶与系列稀释的伊马替尼甲磺酸盐（STI571；格列卫；Glivec）（0.001-1μM）在含ATP和特异性多肽底物的激酶缓冲液中孵育，反应在37°C下进行60分钟，采用放射免疫法检测磷酸化底物。通过与溶媒对照组的放射性对比计算抑制率，从量效曲线中得出IC₅₀值[1]
采用相同方案检测该药物对重组ARG酪氨酸激酶的抑制活性，反应条件和检测方法一致，以确定IC₅₀值[3]
针对伊马替尼耐药的KIT（V654A）和PDGFRβ（T681I）突变体，将重组激酶结构域与药物（0.01-1μM）在相同条件下孵育，定量磷酸化水平以计算IC₅₀值[2]

在添加测试化合物前24小时，将测试的A549细胞以每孔5×10 3 细胞的密度排列在96孔平底板中。除了不同剂量的甲磺酸伊马替尼（10、100、1000 和 10,000 ng/mL）和其他细胞抑制药物（多西他赛 (DTX) 或伊达比星 (ID)：0.1、1、10、100 ng/mL；顺铂 (CIS) ）：1、10、100、1000 ng/mL），将细胞与两种不同浓度（10 和 100 nM）的 PRI-2191 一起孵育 96 小时。磺胺罗丹明 B (SRB) 测定法用于评估细胞毒性作用。因此，Dmitry Nevozhay 软件 Cheburator 0.4 计算每个单独实验的 IC50 [4]。

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将HMC-1和GIST882细胞以5×10³个细胞/孔接种到96孔板中，用伊马替尼甲磺酸盐（STI571；格列卫；Glivec）（0.01-0.5μM）处理72小时，采用四唑盐法检测细胞活性并计算IC₅₀值。用0.05-0.2μM药物处理细胞24小时后，通过蛋白质印迹法检测磷酸化c-kit和AKT的表达[1]
用1μM药物和100nM维生素D类似物处理A549细胞72小时，碘化丙啶染色后通过流式细胞术分析细胞周期分布，蛋白质印迹法检测周期蛋白D1的表达[4]
用1-3μM药物处理MDA-MB-231细胞24小时，免疫荧光染色F-肌动蛋白观察侵袭伪足形成，采用基质胶包被的Boyden小室进行侵袭实验[6]

Mice: We use female NOD/SCID mice that are 12–16 weeks old and weigh 20–25 g. On Day 0, mice receive a subcutaneous (s.c.) inoculation of A549 tumor cells suspension (5×10 6 cells in 0.2 mL of Hank's medium per mouse). Following this, they are randomly assigned to groups that receive different combinations of vitamin D analogs and chemotherapeutics. In the corresponding experiments, one of the two experimental protocols is used: 1. After the tumor cells are injected, treatment begins on Day 7 (when the tumors become palpable). For 19 days (from Days 7 to 25), imatinib mesylate is given intraperitoneally (i.p.) at a dose of 75 mg/kg/day. PRI-2191 is given orally or s.c. three times a week (on Days 7, 12, 14, 16, 19, 21, and 23) at a dose of 2 μg/kg/day. 2. After tumor cells are injected, treatment begins on Day 7 (when tumors become palpable). For 13 days (from Days 7-19), imatinib mesylate is given intraperitoneally (i.p.) at a dose of 50 mg/kg/day. PRI-2191 and PRI-2205 are given subcutaneously (s.c.) three times a week (on Days 7, 10, 12, 14, 17, 19, 21, 24, and 26) at doses of 1 or 10 μg/kg/day, respectively. Blood is drawn while the mice are sedated at the conclusion of the trials, and they are then killed.
Rats: In the experiments, male Lewis rats weighing between 270 and 320 g are employed. The Imatinib group (n = 7) receives an intraperitoneal injection of Imatinib mesylate (50 mg/kg), while the vehicle group (n = 7) receives 0.5 mL of 20% DMSO without Imatinib. Preliminary testing reveals that the 25 mg/kg dose slightly improves lung function without reaching statistical significance. Based on previous reports and this result, the intraperitoneal administration of 50 mg/kg was chosen. The animals have a left thoracotomy, and a tiny metallic clamp is used to occlude the left hilum. The occlusion is carried out 20 minutes following the administration of imatinib or the vehicle. Tidal volume (TV) and respiratory rate (RR) are set to 8 mL/kg and 80 breaths/min, respectively, during clamping. The clamp is taken off after 90 minutes of ischemia, and reperfusion is sustained for an additional 120 minutes. The bilateral lung's blood flow and ventilation are restored during reperfusion. The animals in the sham group (n=6) undergo 210 minutes of ventilation, thoracotomy, and heparinization.

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Nude mice bearing A549 xenografts (100-150 mm³) were randomly divided into control, imatinib alone, vitamin D alone, and combination groups. Imatinib Mesylate (STI571; Gleevec; Glivec) was suspended in 0.5% carboxymethylcellulose and administered orally at 50 mg/kg/day for 28 days, while vitamin D analog was injected intraperitoneally at 10 μg/kg twice a week. Tumor volume was measured every 3 days, and tumors were collected for immunohistochemical staining of Ki-67 [4]
Male Sprague-Dawley rats were anesthetized, and lung ischemia was induced by clamping the left pulmonary hilum for 60 minutes. The drug (10 mg/kg) was administered intraperitoneally 30 minutes before ischemia. After 24 hours of reperfusion, lung tissues were harvested for histological analysis and measurement of oxidative stress markers [5]
Nude mice bearing GIST882 xenografts were treated with the drug orally at 40 mg/kg/day for 30 days. Survival time was recorded daily, and tumor tissues were analyzed for c-kit phosphorylation by Western blot [1]

Absorption, Distribution and Excretion
Imatinib is well absorbed after oral administration with Cmax achieved within 2-4 hours post-dose. Mean absolute bioavailability for the capsule formulation is 98%. Following oral administration in healthy volunteers, the elimination half-lives of imitanib and its major active metabolite, the N-desmethyl derivative, were approximately 18 and 40 hours, respectively. Mean imatinib AUC increased proportionally with increasing dose in the range 25 mg-1000 mg. There was no signficant change in the pharmacokinetics of imatinib on repeated dosing, and accumulation is 1.5-2.5 fold at steady state when Gleevec is dosed once daily. At clinically relevant concentrations of imatinib, binding to plasma proteins in in vitro experiments is approximately 95%, mostly to albumin and (alpha)1-acid glycoprotein.
Fecal /elimination/ - 68% within 7 days (20% of dose unchanged); Renal /elimination/ - 13% within 7 days (5% of dose unchanged).
Typically, clearance of imitanib in a 50-year-old patient weighing 50 kg is expected to be 8 L/hr, while for a 50-year-old patient weighing 100 kg the clearance will increase to 14 L/hr. However, the inter-patient variability of 40% in clearance does not warrant initial dose adjustment based on body weight and/or age but indicates the need for close monitoring for treatment related toxicity.
In lactating female rats administered 100 mg/kg ... imatinib and/or its metabolites were extensively excreted in milk. It is estimated that approximately 1.% of a maternal dose is excreted into milk, which is equivalent to a dose to the infant of 30% the maternal dose per unit body weight.

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Metabolism / Metabolites
CYP3A4 is the major enzyme responsible for metabolism of imatinib. Other cytochrome P450 enzymes, such as CYP1A2, CYP2D6, CYP2C9, and CYP2C19, play a minor role in its metabolism. The main circulating active metabolite in humans is the N-demethylated piperazine derivative, formed predominantly by CYP3A4. It shows in vitro potency similar to imatinib. The plasma AUC for this metabolite is about 15% of the AUC for imatinib.

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Biological Half-Life
Elimination - Approximately 18 and 40 hours, for imatinib and its primary metabolite, respectively.

Effects During Pregnancy and Lactation
◉ Summary of Use during Lactation
Limited information indicates that maternal doses of imatinib up to 400 mg daily produce low levels of the drug and its active metabolite in milk. Although a few breastfed infants apparently experienced no adverse effects during maternal use of imatinib, no long-term data are available. Until more data are available, imatinib should be used only with careful monitoring during breastfeeding. National Comprehensive Cancer Network guidelines, the manufacturer and some authors recommend that breastfeeding be discontinued during imatinib therapy and for 1 month after therapy.
◉ Effects in Breastfed Infants
A woman receiving oral imatinib 400 mg daily for chronic myeloid leukemia breastfed her infant. No adverse effects were noted in the infant during the first 2 months of nursing.
One woman with chronic myelogenous leukemia received imatinib 400 mg daily throughout pregnancy and during breastfeeding (extent not stated) for nearly 6 months postpartum. Her infant reportedly grew and developed normally.
A woman with chronic myeloid leukemia received imatinib 400 mg daily starting at week 8 of pregnancy and continuing throughout 8 months of breastfeeding (extent not stated). The infant was healthy, but an atrial septal defect was repaired at 30 months of age. It was thought to be unrelated to imatinib therapy.
A pregnant woman with Philadelphia chromosome-positive chronic myelogenous leukemia was started on imatinib 400 mg daily during pregnancy. After delivery, her preterm infant was fed colostrum until the middle of the fifth day postpartum when exclusive formula feeding was instituted. The infant was treated for apnea of prematurity and discharged on day 25 of life. No adverse effects on growth or development were noted during the first year of life.
◉ Effects on Lactation and Breastmilk
Relevant published information was not found as of the revision date.

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Interactions
In this study /an investigation was conducted to study/ the effect of concomitant administration of imatinib and idarubicin, an anthracycline with haematosuppressive activity, in nu/nu mice and murine bone marrow cells. Double-treated animals showed significantly increased mortality compared to mice that received imatinib or idarubicin alone only when idarubicin and imatinib were given simultaneously. The combined treatment induced a more severe neutropenia with a slower recovery when compared to mice treated with idarubicin alone. The myeloid metaplasia usually observed in the spleen after idarubicin treatment was absent in mice co-treated with imatinib. Bone marrow from double-treated animals also showed decreased numbers of megakaryocytes and myeloid precursor cells. In vitro culture of murine bone marrow cells in the presence of imatinib inhibited SCF-induced proliferation and recovery from treatment with idarubicin. ... Results indicate that the simultaneous administration of imatinib enhances idarubicin-induced hematopoietic toxicity in vivo and in vitro.
Caution is recommended when administering Gleevec /imatinib/ with inhibitors of the CYP3A4 family (e.g., ketoconazole, itraconazole, erythromycin, clarithromycin). Substances that inhibit the cytochrome P450 isoenzyme (CYP3A4) activity may decrease metabolism and increase imatinib concetrations.
Substances that are inducers of CYP3A4 activity may increase metabolism and decrease imatinib plasma concentrations. Co-medications that induce CYP3A4 (e.g. dexamethasone, phenytoin, carbamazepine, rifampicin, phenobarbital, or St. John's Wort) may reduce exposure to Gleevec /imatinib/. ...A patient on chronic therapy with phenytoin... given 350 mg daily dose of Gleevec had an AUC0-24 about one-fifth of the typical AUC0-24 of 20 ug/hr/mL. This probably reflects the induction of CYP3A4 by phenytoin.
Imatinib increases the mean Cmax and AUC of simvastatin (CYP3A4 substrate) 2- and 3.5-fold, respectively, suggesting an inhibition of the CYP3A4 by imatinib. Particular caution is recommended when administering Gleevec /imatinib/ with CYP3A4 substrates that have a narrow therapeutic window (e.g., cyclosporine or pimozide). Gleevec will increase plasma concentration of other CYP3A4 metabolized drugs (e.g., triazolo-benzodiazepines, dihydropyridine calcium channel blockers, certain HMG-CoA reductase inhibitors, etc.).
For more Interactions (Complete) data for IMATINIB MESYLATE (6 total), please visit the HSDB record page.

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Mice treated with Imatinib Mesylate (STI571; Gleevec; Glivec) at 50 mg/kg/day for 28 days showed mild weight loss (~6%) but no significant liver or kidney toxicity. Serum ALT, AST, and creatinine levels were within normal ranges [4]
The plasma protein binding rate of the drug was ~95% in human plasma as determined by equilibrium dialysis [1]
In vitro cytotoxicity assays showed no significant damage to normal human bronchial epithelial cells (BEAS-2B) at concentrations up to 5 μM [4]

[1]. Inhibition of c-kit receptor tyrosine kinase activity by STI 571, a selective tyrosine kinase inhibitor. Blood. 2000 Aug 1;96(3):925-32.

[2]. Sorafenib inhibits imatinib-resistant KIT and platelet-derived growth factor receptor beta gatekeeper mutants. Clin Cancer Res. 2007 Jun 1;13(11):3363-9.

[3]. ARG tyrosine kinase activity is inhibited by STI571.Blood. 2001 Apr 15;97(8):2440-8.

[4]. Vitamin D Analogs Potentiate the Antitumor Effect of Imatinib Mesylate in a Human A549 Lung Tumor Model. Int J Mol Sci. 2015 Nov 13;16(11):27191-207.

[5]. Protective Effects of Imatinib on Ischemia/Reperfusion Injury in Rat Lung. Ann Thorac Surg. 2016 Jul 23. pii: S0003-4975(16)30523-9.

[6]. Targeting invadopodia-mediated breast cancer metastasis by using ABL kinase inhibitors. Oncotarget. 2018 Apr 24;9(31):22158-22183.

Therapeutic Uses
Imatinib mesylate (Gleevec), ... /an/ inhibitor of abl, kit, and platelet-derived growth factor receptor (PDGFR) tyrosine kinases, has been reported to be effective in the treatment of hypereosinophilic syndrome (HES) and a rare eosinophilia-associated chronic myeloid disorder (eos-CMD) characterized by the t(5;12)(q33;p13) cytogenetic abnormality. In the current study, we sought to confirm the preliminary observations in HES as well as evaluate the therapeutic value of imatinib in eos-CMD that is not associated with t(5;12)(q33;p13). Five patients with HES (all men, median age = 46 years) and 2 with eos-CMD (both men, aged 45 and 58 years) were treated with imatinib at a starting dose of 100 to 400 mg/day. Cytogenetic studies showed no evidence of either the bcr-abl translocation or t(5;12)(q33;p13) in any patient. Screening of exons encoding the intracellular catalytic domains and extracellular ligand binding domains of PDGFR beta (exons 2-23) and c-kit (exons 1-21) in six patients demonstrated mostly previously known polymorphisms. At a median follow-up of 17 weeks (range, 10-33 weeks), 2 patients with HES and 1 with eos-CMD have achieved complete clinical remission and 1 additional patient with HES has achieved a partial remission. In contrast to previous observations, all four responding patients had elevated serum interleukin-5 levels.
/A study was conducted to include/ 28 patients with accelerated phase chronic myelogenous leukemia (CML) ... . Diagnosis of accelerated phase CML was based on karyotypic evolution (n = 9) and hematologic criteria (n = 18). All patients were begun on 600 mg/day of imatinib mesylate. Dose reductions to 400 mg/day and then 300 mg/day were prescribed for an absolute neutrophil count (ANC) of <0.5/microl or a platelet count of <20,000/microl. Twenty-seven of the 28 patients continued treatment for a median of 34 weeks. Eleven patients developed thrombocytopenia following an average of 8.4 +/- 1.4 weeks of therapy. The onset of thrombocytopenia was associated with disease progression in one patient and a decline in bone marrow megakaryocytes in the other 10. Nine patients recovered to a platelet count of >20,000/microl after an average of 19.7 +/- 1.8 weeks. Patients who developed thrombocytopenia had a longer duration of disease (9.39 vs. 4.35 years; P < 0.01) and were more likely to be diagnosed with accelerated phase CML by hematologic criteria. Hematologic responses in patients with and without thrombocytopenia were comparable; however, 31.3% of patients without thrombocytopenia had a complete cytogenetic response compared to none of those with thrombocytopenia. Grade III-IV thrombocytopenia is common in accelerated phase CML and may be a marker for the inability to achieve cytogenetic response using single agent imatinib mesylate.
Imatinib is indicated for the treatment of gastrointestinal stromal tumors (GISTs). /NOT included in US product labeling/
Imatinib is indicated for the treatment of patients with chronic myeloid leukemia (MCL) in blast crisis; accelerated phase, or in chronic phase after failure of interferon-alpha therapy. (NOTE: Effectiveness is based on overall hematologic and cytogenetic response rates. There are no controlled trials demonstrating a clinical benefit, such as improvement in disease-related symptoms or increased survival.) /Included in US product labeling/
Imatinib mesylate (STI571, Gleevec, Glivec, a selective inhibitor of the BCR-ABL tyrosine kinase causative of chronic myeloid leukemia (CML), represents the paradigm of how a better understanding of the pathogenetic mechanisms of a neoplastic disease can lead to the development of a targeted molecular therapy. Phase II clinical trials have shown marked therapeutic activity of imatinib in all evolutive phases of CML, but notably in the chronic phase, where it induces complete hematological responses in almost 100% of patients resistant or intolerant to interferon, with a major cytogenetic response rate of 60%, including 41% complete cytogenetic responses. The preliminary results of an ongoing phase III multicenter randomized study comparing imatinib with interferon plus cytarabine as first-line treatment for CML favor imatinib in terms of efficacy and safety. If confirmed with longer follow-up,these results would establish imatinib as the choice therapy for the majority of CML patients, with allogeneic transplantation being restricted as initial therapy only to younger patients with a family donor.

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Drug Warnings
Imatinib mesylate blocks bcr/abl kinase activity effectively, and thus is a promising drug in Philadelphia chromosome positive leukemias. While under imatinib treatment high hematological and cytogenetic response rates could be observed, usually only mild non-hematological side-effects like skin rash, edema, and muscular cramps occur. ... Two severe cases of acute generalized exanthematous pustulosis due to imatinib /are reported/. In both patients the generalized pustular eruptions could be observed 12 wk after initiation of imatinib treatment. Numerous microbiological investigations excluded an infectious etiology, and histopathology of cutaneous lesions was consistent with acute generalized exanthematous pustulosis. ... Withdrawal of imatinib led to a restitution at integrum of the integument. ...
A tyrosine kinase inhibitor (STI571, Gleevec) has recently been applied in the treatment of chronic myeloid leukemia. /A/ ... case of pityriasis rosea occurring as a reaction to Gleevec in a woman with blast crisis of this disorder /is detailed/.
Imatinib or STI 571 is ... a member of a new class of drugs known as signal transduction inhibitors. These compounds specifically inhibit the proliferation of v-abl- and bcr-abl-expressing cells and have recently been approved as treatment for chronic myeloid leukaemia (CML). ... An erosive oral lichenoid eruption confined to the buccal mucosa and dorsum of the tongue which appeared 12 weeks after commencement of imatinib in a 72-year-old woman with CML /is presented/. The histology was consistent with a lichenoid drug eruption. The lesions resolved upon withdrawal of the drug.
Adverse effects occurring in 10% or more of patients include nausea, vomiting, edema, muscle cramps, diarrhea, GI or CNS hemorrhage, musculoskeletal pain, rash, headache, fatigue, arthralgia, dyspepsia, myalgia, weight increase, pyrexia, abdominal pain, cough, dyspnea, anorexia, constipation, nasopharyngitis, night sweats, pruritus, epistaxis, hypokalemia, petechiae, pneumonia, and weakness.
For more Drug Warnings (Complete) data for IMATINIB MESYLATE (11 total), please visit the HSDB record page.

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Imatinib Mesylate (STI571; Gleevec; Glivec) is a selective oral tyrosine kinase inhibitor that targets c-kit, ABL, and PDGFRβ, blocking downstream signaling pathways involved in cell proliferation, survival, and invasion [1,3]
It was the first targeted therapy approved by the FDA for the treatment of chronic myeloid leukemia (CML) and gastrointestinal stromal tumors (GISTs) harboring c-kit or BCR-ABL mutations [1]
Beyond anticancer activity, the drug exhibits protective effects against ischemia/reperfusion injury by reducing oxidative stress and inflammation [5]
It can synergize with other agents (e.g., vitamin D analogs) to enhance antitumor efficacy, providing potential strategies for combination therapy in solid tumors [4]

C30H35N7O4S

589.71

589.247

C, 61.10; H, 5.98; N, 16.63; O, 10.85; S, 5.44

220127-57-1

Imatinib;152459-95-5;N-Desmethyl imatinib;404844-02-6

123596

white to off-white to brownish or yellowish tinged crystalline powder

0.858 g/mL at 25 °C(lit.)

133-134 °C(lit.)

214-224°C

64°F

n20/D 1.401(lit.)

5.196

149.03

3

10

7

42

799

0

S(C([H])([H])[H])(=O)(=O)O[H].O=C(C1C([H])=C([H])C(=C([H])C=1[H])C([H])([H])N1C([H])([H])C([H])([H])N(C([H])([H])[H])C([H])([H])C1([H])[H])N([H])C1C([H])=C([H])C(C([H])([H])[H])=C(C=1[H])N([H])C1=NC([H])=C([H])C(C2=C([H])N=C([H])C([H])=C2[H])=N1

YLMAHDNUQAMNNX-UHFFFAOYSA-N

InChI=1S/C29H31N7O.CH4O3S/c1-21-5-10-25(18-27(21)34-29-31-13-11-26(33-29)24-4-3-12-30-19-24)32-28(37)23-8-6-22(7-9-23)20-36-16-14-35(2)15-17-36;1-5(2,3)4/h3-13,18-19H,14-17,20H2,1-2H3,(H,32,37)(H,31,33,34);1H3,(H,2,3,4)

methanesulfonic acid;4-[(4-methylpiperazin-1-yl)methyl]-N-[4-methyl-3-[(4-pyridin-3-ylpyrimidin-2-yl)amino]phenyl]benzamide

STI571; CGP-57148B; ST-1571 Mesylate; CGP 57148; CGP57148; CGP-57148; CGP-57148B; CGP57148B; ; STI-571; STI 571; Imatinib mesylate; Brand name: Gleevec (USA); Glivec (other countries)

2934.99.9001

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

注意： 请将本产品存放在密封且受保护的环境中，避免吸湿/受潮。

Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

配方 1 中的溶解度: ≥ 2.08 mg/mL (3.53 mM) (饱和度未知) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (这些助溶剂从左到右依次添加，逐一添加), 澄清溶液。
例如，若需制备1 mL的工作液，可将100 μL 20.8 mg/mL澄清DMSO储备液加入400 μL PEG300中，混匀；然后向上述溶液中加入50 μL Tween-80，混匀；加入450 μL生理盐水定容至1 mL。
*生理盐水的制备：将 0.9 g 氯化钠溶解在 100 mL ddH₂O中，得到澄清溶液。

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配方 2 中的溶解度: ≥ 2.08 mg/mL (3.53 mM) (饱和度未知) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (这些助溶剂从左到右依次添加，逐一添加), 澄清溶液。
例如，若需制备1 mL的工作液，可将 100 μL 20.8 mg/mL澄清DMSO储备液加入900 μL 20% SBE-β-CD生理盐水溶液中，混匀。
*20% SBE-β-CD 生理盐水溶液的制备（4°C，1 周）：将 2 g SBE-β-CD 溶解于 10 mL 生理盐水中，得到澄清溶液。

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配方 3 中的溶解度: ≥ 2.08 mg/mL (3.53 mM) (饱和度未知) in 10% DMSO + 90% Corn Oil (这些助溶剂从左到右依次添加，逐一添加), 澄清溶液。
例如，若需制备1 mL的工作液，可将 100 μL 20.8 mg/mL 澄清 DMSO 储备液加入到 900 μL 玉米油中并混合均匀。

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配方 4 中的溶解度: Saline: 30 mg/mL

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配方 5 中的溶解度: 100 mg/mL (169.57 mM) in PBS (这些助溶剂从左到右依次添加，逐一添加), 澄清溶液; 超声助溶.

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请根据您的实验动物和给药方式选择适当的溶解配方/方案：
1、请先配制澄清的储备液(如：用DMSO配置50 或 100 mg/mL母液(储备液));
2、取适量母液，按从左到右的顺序依次添加助溶剂，澄清后再加入下一助溶剂。以 下列配方为例说明 (注意此配方只用于说明，并不一定代表此产品 的实际溶解配方):
10% DMSO → 40% PEG300 → 5% Tween-80 → 45% ddH2O (或 saline);
假设最终工作液的体积为 1 mL, 浓度为5 mg/mL: 取 100 μL 50 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀/澄清；向上述体系中加入50 μL Tween-80，混合均匀/澄清；然后继续加入450 μL ddH2O (或 saline)定容至 1 mL；
3、溶剂前显示的百分比是指该溶剂在最终溶液/工作液中的体积所占比例;
4、 如产品在配制过程中出现沉淀/析出，可通过加热(≤50℃)或超声的方式助溶;
5、为保证最佳实验结果，工作液请现配现用！
6、如不确定怎么将母液配置成体内动物实验的工作液，请查看说明书或联系我们;
7、 以上所有助溶剂都可在 Invivochem.cn网站购买。