丁苯那嗪（皮下注射，1~10 mg/kg，一次）可剂量依赖性降低成年雄性小鼠神经递质分子NE、DA、5-HT的水平[1]。在雄性ICR小鼠中，丁苯那嗪（腹腔注射，0-2 mg/kg，一次）选择性地影响运动，可以大大减少吗啡引起的多动症，尽管它也会引起口腔震颤和刻板行为[2]。丁苯那嗪（ip，0.25-2 mg/kg，每周一次）可剂量依赖性地增加成年雄性 Sprague-Dawley 大鼠的震颤下颌运动 (TJM) [3]。

Animal/Disease Models: Adult male MAO A KO or 1-2 month old wide-type mice [1]
Doses: 1-10 mg/kg
Route of Administration: subcutaneous injection; one-time
Experimental Results:complete elimination of aggressive behavior at a concentration of 5 mg/kg, and Dramatically diminished their NE, DA and 5-HT levels.

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Animal/Disease Models: Male ICR mice (10 weeks old) [2]
Doses: 0-2 mg/kg
Route of Administration: intraperitoneal (ip) injection;
Experimental Results: Pretreatment with tetrabenazine diminished morphine-induced hyperactivity. The METH-induced increase in locomotion was diminished at the 1 mg/kg dose.

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Animal/Disease Models: Adult male SD (SD (Sprague-Dawley)) rats, body weight 350-450 g[3]
Doses: 0.25-2 mg/kg
Route of Administration: intraperitoneal (ip) injection; intraperitoneal (ip) injection. Weekly
Experimental Results: Dramatically induced jaw tremor (TJM) at 2 mg/kg, with more motor injuries seen at higher doses (e.g. 3-4 mg/kg).

[1]. J C Shih, et al. Ketanserin and tetrabenazine abolish aggression in mice lacking monoamine oxidase A. Brain Res. 1999 Jul 24;835(2):104-12.
[2]. Nobue Kitanaka, et al. Tetrabenazine, a vesicular monoamine transporter-2 inhibitor, attenuates morphine-induced hyperlocomotion in mice through alteration of dopamine and 5-hydroxytryptamine turnover in the cerebral cortex. Pharmacol Biochem Behav. 2018
[3]. S J Podurgiel, et al. The vesicular monoamine transporter (VMAT-2) inhibitor tetrabenazine induces tremulous jaw movements in rodents: implications for pharmacological models of parkinsonian tremor. Neuroscience. 2013 Oct 10;250:507-19. doi: 10.1016/j.neu
[4]. Kenney, C., C. Hunter, and J. Jankovic, Long-term tolerability of tetrabenazine in the treatment of hyperkinetic movement disorders. Mov Disord, 2007. 22(2): p. 193-7.
[5]. Jankovic, J. and J. Beach, Long-term effects of tetrabenazine in hyperkinetic movement disorders. Neurology, 1997. 48(2): p. 358-62.
[6]. Ondo, W.G., P.A. Hanna, and J. Jankovic, Tetrabenazine treatment for tardive dyskinesia: assessment by randomized videotape protocol. Am J Psychiatry, 1999. 156(8): p. 1279-81.

C19H27NO3

317.42

58-46-8

Tetrabenazine Racemate;718635-93-9;(+)-Tetrabenazine;1026016-83-0;Tetrabenazine-d6;1392826-25-3;Tetrabenazine mesylate;804-53-5;(+)-Tetrabenazine-d6;1977511-05-9

O=C1[C@@H](CC(C)C)CN2CCC3=CC(OC)=C(OC)C=C3[C@]2([H])C1

MKJIEFSOBYUXJB-HOCLYGCPSA-N

InChI=1S/C19H27NO3/c1-12(2)7-14-11-20-6-5-13-8-18(22-3)19(23-4)9-15(13)16(20)10-17(14)21/h8-9,12,14,16H,5-7,10-11H2,1-4H3/t14-,16-/m0/s1

(3S,11bS)-3-isobutyl-9,10-dimethoxy-1,3,4,6,7,11b-hexahydro-2H-pyrido[2,1-a]isoquinolin-2-one

Tetrabenazine; trade names Nitoman and Xenazin; Ro 1 9569; Ro-1 9569; Ro 1-9569

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

配方 1 中的溶解度: ≥ 2.5 mg/mL (7.88 mM) (饱和度未知) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (这些助溶剂从左到右依次添加，逐一添加), 澄清溶液。
例如，若需制备1 mL的工作液，可将100 μL 25.0 mg/mL澄清DMSO储备液加入到400 μL PEG300中，混匀；然后向上述溶液中加入50 μL Tween-80，混匀；加入450 μL生理盐水定容至1 mL。
*生理盐水的制备：将 0.9 g 氯化钠溶解在 100 mL ddH₂O中，得到澄清溶液。

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配方 2 中的溶解度: ≥ 2.5 mg/mL (7.88 mM) (饱和度未知) in 10% DMSO + 90% Corn Oil (这些助溶剂从左到右依次添加，逐一添加), 澄清溶液。
例如，若需制备1 mL的工作液，可将 100 μL 25.0 mg/mL 澄清 DMSO 储备液加入到 900 μL 玉米油中并混合均匀。

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请根据您的实验动物和给药方式选择适当的溶解配方/方案：
1、请先配制澄清的储备液(如：用DMSO配置50 或 100 mg/mL母液(储备液));
2、取适量母液，按从左到右的顺序依次添加助溶剂，澄清后再加入下一助溶剂。以 下列配方为例说明 (注意此配方只用于说明，并不一定代表此产品 的实际溶解配方):
10% DMSO → 40% PEG300 → 5% Tween-80 → 45% ddH2O (或 saline);
假设最终工作液的体积为 1 mL, 浓度为5 mg/mL: 取 100 μL 50 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀/澄清；向上述体系中加入50 μL Tween-80，混合均匀/澄清；然后继续加入450 μL ddH2O (或 saline)定容至 1 mL；
3、溶剂前显示的百分比是指该溶剂在最终溶液/工作液中的体积所占比例;
4、 如产品在配制过程中出现沉淀/析出，可通过加热(≤50℃)或超声的方式助溶;
5、为保证最佳实验结果，工作液请现配现用！
6、如不确定怎么将母液配置成体内动物实验的工作液，请查看说明书或联系我们;
7、 以上所有助溶剂都可在 Invivochem.cn网站购买。