- NADP acts as a cofactor binding to NADP-dependent enzymes, including dehydrogenases (e.g., glucose-6-phosphate dehydrogenase), reductases (e.g., glutathione reductase), and oxidases[2]

- 研发了一种基因编码的NADP+生物传感器（源于NADP+结合蛋白）。体外实验中，该传感器对NADP+的荧光响应线性范围为0.1 μM至100 μM，特异性高（不与NAD+、ATP或GTP交叉反应）。当NADP+浓度从0.1 μM升至100 μM时，传感器荧光信号增强约5倍，可实现缓冲液中NADP+的定量检测[1]
- 纯化重组NADP依赖性酶（如苹果酸脱氢酶），通过X射线晶体学分析其与NADP的结合。结果证实，NADP通过保守氢键和疏水相互作用结合于酶的活性位点，其中NADP的2'-磷酸基团起关键作用——可将其与无磷酸基团的NAD+区分开，确保特异性结合[2]

- NADP+生物传感器在斑马鱼胚胎和成年小鼠中表达。在斑马鱼中，荧光成像显示葡萄糖刺激下肝脏和肌肉组织中NADP+水平动态变化（刺激后30分钟NADP+增加约2倍）；在小鼠中，腹腔注射丙酮酸可诱导肾皮质中NADP+水平增加约1.5倍，该变化可通过生物传感器检测到[1]

- 细胞内NADP+检测流程：通过脂质体转染将生物传感器质粒导入HeLa细胞，培养24小时后，用代谢抑制剂（如6-氨基烟酰胺，一种NADP依赖性酶抑制剂）处理细胞。使用共聚焦显微镜捕获荧光图像，量化荧光强度以计算细胞内NADP+浓度。实验显示，与对照组相比，6-氨基烟酰胺处理使细胞内NADP+水平降低约40%[1]

[1]. A genetically encoded biosensor for in vitro and in vivo detection of NADP+. Biosens Bioelectron. 2016 Mar 15;77:901-6.

[2]. NADP-dependent enzymes. I: Conserved stereochemistry of cofactor binding. Proteins. 1997 May;28(1):10-28.

[3]. The phosphate makes a difference: cellular functions of NADP. Redox Rep. 2010;15(1):2-10.

NADP zwitterion is a NADP. It has a role as a fundamental metabolite. It is a conjugate base of a NADP(+).
Nicotinamide adenine dinucleotide phosphate. A coenzyme composed of ribosylnicotinamide 5'-phosphate (NMN) coupled by pyrophosphate linkage to the 5'-phosphate adenosine 2',5'-bisphosphate. It serves as an electron carrier in a number of reactions, being alternately oxidized (NADP+) and reduced (NADPH). (Dorland, 27th ed)
NADP is a metabolite found in or produced by Escherichia coli (strain K12, MG1655).
Triphosphopyridine nucleotide has been reported in Arabidopsis thaliana, Homo sapiens, and other organisms with data available.
NADP is nicotinamide Adenine Dinucleotide Phosphate, an oxidation-reduction coenzyme, found in eukaryotic cells and is involved in many enzymatic reactions.
Nicotinamide adenine dinucleotide phosphate. A coenzyme composed of ribosylnicotinamide 5'-phosphate (NMN) coupled by pyrophosphate linkage to the 5'-phosphate adenosine 2',5'-bisphosphate. It serves as an electron carrier in a number of reactions, being alternately oxidized (NADP+) and reduced (NADPH). (Dorland, 27th ed)

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- NADP (nicotinamide adenine dinucleotide phosphate) exists in two forms: oxidized (NADP+) and reduced (NADPH). It is an essential cofactor in cellular redox reactions, participating in processes such as lipid synthesis, nucleotide synthesis, and antioxidant defense (e.g., regenerating glutathione via glutathione reductase using NADPH) [3]
- The 2'-phosphate group of NADP is critical for its biological function: it mediates specific binding to NADP-dependent enzymes (preventing binding to NAD-dependent enzymes) and regulates the enzyme’s catalytic activity by inducing conformational changes in the enzyme’s active site [2]
- The genetically encoded NADP+ biosensor enables real-time, non-invasive detection of NADP+ levels in living cells and organisms, providing a tool for studying NADP-related metabolic pathways and diseases (e.g., metabolic disorders associated with NADP imbalance) [1]
- In cells, NADP is primarily localized in the cytoplasm, mitochondria, and chloroplasts (in plants). The cytoplasmic NADPH/NADP+ ratio is maintained at ~100:1 to support reductive biosynthesis, while the mitochondrial ratio is lower (~7:1) to balance oxidative reactions [3]

C21H28N7O17P3

743.41

743.075

53-59-8

53-57-6 (reduced);604-79-5 (oxidized);53-59-8 (free);1184-16-3 (Na); 100929-71-3 (ammonium); 24294-60-2 (disodium);

5885

White to off-white solid powder

-7.3

397.05

8

21

13

48

1290

8

C1=CC(=C[N+](=C1)[C@H]2[C@@H]([C@@H]([C@H](O2)COP(=O)([O-])OP(=O)(O)OC[C@@H]3[C@H]([C@H]([C@@H](O3)N4C=NC5=C(N=CN=C54)N)OP(=O)(O)O)O)O)O)C(=O)N

XJLXINKUBYWONI-NNYOXOHSSA-N

InChI=1S/C21H28N7O17P3/c22-17-12-19(25-7-24-17)28(8-26-12)21-16(44-46(33,34)35)14(30)11(43-21)6-41-48(38,39)45-47(36,37)40-5-10-13(29)15(31)20(42-10)27-3-1-2-9(4-27)18(23)32/h1-4,7-8,10-11,13-16,20-21,29-31H,5-6H2,(H7-,22,23,24,25,32,33,34,35,36,37,38,39)/t10-,11-,13-,14-,15-,16-,20-,21-/m1/s1

[[(2R,3R,4R,5R)-5-(6-aminopurin-9-yl)-3-hydroxy-4-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(2R,3S,4R,5R)-5-(3-carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate

2934.99.9001

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

注意： 本产品在运输和储存过程中需避光。

Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

H2O : ~250 mg/mL (~336.29 mM)
DMSO :< 1 mg/mL

配方 1 中的溶解度: 100 mg/mL (134.52 mM) in PBS (这些助溶剂从左到右依次添加，逐一添加), 澄清溶液; 超声助溶。

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请根据您的实验动物和给药方式选择适当的溶解配方/方案：
1、请先配制澄清的储备液(如：用DMSO配置50 或 100 mg/mL母液(储备液));
2、取适量母液，按从左到右的顺序依次添加助溶剂，澄清后再加入下一助溶剂。以 下列配方为例说明 (注意此配方只用于说明，并不一定代表此产品 的实际溶解配方):
10% DMSO → 40% PEG300 → 5% Tween-80 → 45% ddH2O (或 saline);
假设最终工作液的体积为 1 mL, 浓度为5 mg/mL: 取 100 μL 50 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀/澄清；向上述体系中加入50 μL Tween-80，混合均匀/澄清；然后继续加入450 μL ddH2O (或 saline)定容至 1 mL；
3、溶剂前显示的百分比是指该溶剂在最终溶液/工作液中的体积所占比例;
4、 如产品在配制过程中出现沉淀/析出，可通过加热(≤50℃)或超声的方式助溶;
5、为保证最佳实验结果，工作液请现配现用！
6、如不确定怎么将母液配置成体内动物实验的工作液，请查看说明书或联系我们;
7、 以上所有助溶剂都可在 Invivochem.cn网站购买。