规格 | 价格 | 库存 | 数量 |
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1mg |
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2mg |
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5mg |
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10mg |
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25mg |
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50mg |
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100mg |
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Other Sizes |
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体外研究 (In Vitro) |
LDN193189 (GMP)(250 nM;0-7 天)刺激人多能干细胞 (hPSC) 直接发育为中脑多巴胺神经元 (mDA)[1]。在体外,浓度为 200 nM 的 LDN193189 (GMP) 可诱导 hPSC 产生葡萄糖反应性 β 细胞 [2]。
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体内研究 (In Vivo) |
LDN-193189(腹腔注射;3 mg/kg;每天;35 天)可能会影响乳腺癌细胞与其周围骨骼的相互作用[3]。 LDN-193189(腹腔注射;3 mg/kg;单剂量)可减少功能损伤和异位骨化 [1]。
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动物实验 |
Animal/Disease Models: Ahymic NMRI nude female mice (6weeks old)[3]
Doses: 3 mg/kg Route of Administration: Itraperitoneal, daily, for 35 days Experimental Results: Ehanced etastases development in vivo. Animal/Disease Models: C57BL/6 mice[1] Doses: 3 mg/kg Route of Administration: Intraperitoneal, single Experimental Results: Diminished ectopic bone formation and preserved joint spaces over the same interval without inducing fractures, osteopenia or skeletal abnormalities. |
参考文献 |
[1]. Kim TW, et al. Biphasic Activation of WNT Signaling Facilitates the Derivation of Midbrain Dopamine Neurons from hESCs for Translational Use. Cell Stem Cell. 2021 Feb 4;28(2):343-355.e5.
[2]. Pagliuca FW, et al. Generation of functional human pancreatic β cells in vitro. Cell. 2014 Oct 9;159(2):428-39. |
分子式 |
C25H22N6
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分子量 |
406.48
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CAS号 |
1062368-24-4
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相关CAS号 |
LDN193189;1062368-24-4
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SMILES |
C1(C2=C3N=CC(C4=CC=C(N5CCNCC5)C=C4)=CN3N=C2)=CC=NC6=CC=CC=C16
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化学名 |
4-[6-(4-Piperazin-1-yl-phenyl)-pyrazolo[1,5-a]pyrimidin-3-yl]-quinoline
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别名 |
DM3189; LDN193189; DM-3189; LDN 193189; DM 3189; LDN-193189
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存储方式 |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
运输条件 |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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溶解度 (体外) |
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溶解度 (体内) |
Solubility in Formulation 1: ≥ 1.2 mg/mL (2.95 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.2 mg/mL (2.95 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: Saline (suspension): 30 mg/mL 1、请先配制澄清的储备液(如:用DMSO配置50 或 100 mg/mL母液(储备液)); 2、取适量母液,按从左到右的顺序依次添加助溶剂,澄清后再加入下一助溶剂。以 下列配方为例说明 (注意此配方只用于说明,并不一定代表此产品 的实际溶解配方): 10% DMSO → 40% PEG300 → 5% Tween-80 → 45% ddH2O (或 saline); 假设最终工作液的体积为 1 mL, 浓度为5 mg/mL: 取 100 μL 50 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀/澄清;向上述体系中加入50 μL Tween-80,混合均匀/澄清;然后继续加入450 μL ddH2O (或 saline)定容至 1 mL; 3、溶剂前显示的百分比是指该溶剂在最终溶液/工作液中的体积所占比例; 4、 如产品在配制过程中出现沉淀/析出,可通过加热(≤50℃)或超声的方式助溶; 5、为保证最佳实验结果,工作液请现配现用! 6、如不确定怎么将母液配置成体内动物实验的工作液,请查看说明书或联系我们; 7、 以上所有助溶剂都可在 Invivochem.cn网站购买。 |
制备储备液 | 1 mg | 5 mg | 10 mg | |
1 mM | 2.4601 mL | 12.3007 mL | 24.6015 mL | |
5 mM | 0.4920 mL | 2.4601 mL | 4.9203 mL | |
10 mM | 0.2460 mL | 1.2301 mL | 2.4601 mL |
1、根据实验需要选择合适的溶剂配制储备液 (母液):对于大多数产品,InvivoChem推荐用DMSO配置母液 (比如:5、10、20mM或者10、20、50 mg/mL浓度),个别水溶性高的产品可直接溶于水。产品在DMSO 、水或其他溶剂中的具体溶解度详见上”溶解度 (体外)”部分;
2、如果您找不到您想要的溶解度信息,或者很难将产品溶解在溶液中,请联系我们;
3、建议使用下列计算器进行相关计算(摩尔浓度计算器、稀释计算器、分子量计算器、重组计算器等);
4、母液配好之后,将其分装到常规用量,并储存在-20°C或-80°C,尽量减少反复冻融循环。
计算结果:
工作液浓度: mg/mL;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL)。如该浓度超过该批次药物DMSO溶解度,请首先与我们联系。
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL ddH2O,混匀澄清。
(1) 请确保溶液澄清之后,再加入下一种溶剂 (助溶剂) 。可利用涡旋、超声或水浴加热等方法助溶;
(2) 一定要按顺序加入溶剂 (助溶剂) 。
Inhibitor binding to ActRII.J Biol Chem.2015 Feb 6;290(6):3390-404. Dorsomorphin and LDN-193189 inhibit GDF8-induced signaling pathways in undifferentiated and in differentiated primary human myoblasts and in C2C12 premyoblasts.J Biol Chem.2015 Feb 6;290(6):3390-404. th> |
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Ligand-specific effects of kinase inhibitors on Smad2/3 and Smad1/5 phosphorylation. Dorsomorphin treatment facilitates myotube formation.J Biol Chem.2015 Feb 6;290(6):3390-404. td> |
Dorsomorphin and LDN-193189 efficiently inhibit GDF8 induced Smad3/4 reporter gene activity.J Biol Chem.2015 Feb 6;290(6):3390-404. td> |
Dorsomorphin and LDN-193189 counteract GDF8-induced repression of myogenic differentiation.J Biol Chem.2015 Feb 6;290(6):3390-404. th> |
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Dorsomorphin and LDN-193189 promote the formation of a contractile myotube network.J Biol Chem.2015 Feb 6;290(6):3390-404. td> |