mERα (IC50 = 2.3 nM); hERα (IC50 =28 nM)

苯甲酸雌二醇是天然雌激素雌二醇的合成酯，更准确地说是其 3-苯甲酰酯。胚胎发生和青春期期间女性基因型的发育受到雌激素的调节。绝经前卵巢分泌的主要雌激素是雌二醇。为了诱导家畜发情，苯甲酸雌二醇（一种在 C-3 位具有苄酯的雌二醇类似物）经常与孕激素联合使用。该化合物的 IC50 值在 22 至 28 nM 之间，可与人类、小鼠和鸡的雌激素受体 α (ERα) 结合。与雌二醇相比，这表明结合亲和力降低了 6-10 倍。[1]

在 Ovx 女性模型中，苯甲酸雌二醇（20-100 μg/kg，皮下注射，每天一次，持续 4-5 周）可增强学习和记忆行为 [1]。苯甲酸雌二醇（皮下注射，单剂量，0.015–15000 μg/kg）

Animal/Disease Models: Ovx mouse [1]
Doses: 20, 100 or 200 μg/kg
Route of Administration: subcutaneous injection
Experimental Results: Significant It reverses Ovx-induced decrease in uterine weight. There was no significant effect on the locomotor activity of Ovx mice. Effectively reverses Ovx-induced changes in synaptic cleft enlargement and PSD thickness reduction. Replacement with EB (100 μg/kg) for 4 weeks increased the number and density of synaptic vesicles.

[1]. Xu X, et al. Effects of estradiol benzoate on learning-memory behavior and synaptic structure in ovariectomized mice [J]. Life sciences, 2006, 79(16): 1553-1560.
[2]. Putz O, et al. Neonatal low-and high-dose exposure to estradiol benzoate in the male rat: I. Effects on the prostate gland [J]. Biology of reproduction, 2001, 65(5): 1496-1505.
[3]. He J, et al. Identification of Estradiol Benzoate as an Inhibitor of HBx Using Inducible Stably Transfected HepG2 Cells Expressing HiBiT Tagged HBx [J]. Molecules, 2022, 27(15): 5000.
[4]. Lu D, et al. Investigation of Antiparasitic Activity of Two Marine Natural Products, Estradiol Benzoate, and Octyl Gallate, on Toxoplasma gondii In Vitro [J]. Frontiers in Pharmacology, 2022, 13: 841941.
[5]. Rao M V, et al. Effect of estradiol benzoate on reproductive organs and fertility in the male rat [J]. European Journal of Obstetrics & Gynecology and Reproductive Biology, 1983, 15(3): 189-198.
[6]. Zovko M, et al. Macromolecular prodrugs XI. Synthesis and characterization of polymer-estradiol conjugate. Int J Pharm. 2004 Nov 5;285(1-2):35-41.
[7]. García-Gómez E, et al. Role of sex steroid hormones in bacterial-host interactions. Biomed Res Int. 2013;2013:928290.
[8]. Estradiol benzoate

C25H28O3

376.49

376.2038

C, 79.76; H, 7.50; O, 12.75

50-50-0

Estradiol;50-28-2;Estradiol (cypionate);313-06-4;Estradiol benzoate-d3;1192354-74-7;Estradiol enanthate;4956-37-0

Solid powder

C[C@]12CC[C@H]3[C@H]([C@@H]1CC[C@@H]2O)CCC4=C3C=CC(=C4)OC(=O)C5=CC=CC=C5

UYIFTLBWAOGQBI-BZDYCCQFSA-N

InChI=1S/C25H28O3/c1-25-14-13-20-19-10-8-18(28-24(27)16-5-3-2-4-6-16)15-17(19)7-9-21(20)22(25)11-12-23(25)26/h2-6,8,10,15,20-23,26H,7,9,11-14H2,1H3/t20-,21-,22+,23+,25+/m1/s1

[(8R,9S,13S,14S,17S)-17-hydroxy-13-methyl-6,7,8,9,11,12,14,15,16,17-decahydrocyclopenta[a]phenanthren-3-yl] benzoate

Estradiol monobenzoate; Benzo-Gynoestryl; Ovasterol-B; Oestradiol benzoate; Estradiol Benzoate

2934.99.9001

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

DMSO: 19~100 mg/mL (265.61~50.5 mM)
H2O: ~0.1 mg/mL (~0.3 mM)

Solubility in Formulation 1: ≥ 2.5 mg/mL (6.64 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.

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请根据您的实验动物和给药方式选择适当的溶解配方/方案：
1、请先配制澄清的储备液(如：用DMSO配置50 或 100 mg/mL母液(储备液));
2、取适量母液，按从左到右的顺序依次添加助溶剂，澄清后再加入下一助溶剂。以 下列配方为例说明 (注意此配方只用于说明，并不一定代表此产品 的实际溶解配方):
10% DMSO → 40% PEG300 → 5% Tween-80 → 45% ddH2O (或 saline);
假设最终工作液的体积为 1 mL, 浓度为5 mg/mL: 取 100 μL 50 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀/澄清；向上述体系中加入50 μL Tween-80，混合均匀/澄清；然后继续加入450 μL ddH2O (或 saline)定容至 1 mL；
3、溶剂前显示的百分比是指该溶剂在最终溶液/工作液中的体积所占比例;
4、 如产品在配制过程中出现沉淀/析出，可通过加热(≤50℃)或超声的方式助溶;
5、为保证最佳实验结果，工作液请现配现用！
6、如不确定怎么将母液配置成体内动物实验的工作液，请查看说明书或联系我们;
7、 以上所有助溶剂都可在 Invivochem.cn网站购买。