Human Endogenous Metabolite

Octocadecane（12.5-100 μg/ml；18 小时）对 B16F10-Nex2 细胞表现出高细胞毒作用，IC50 值为 41.08 μg/ml[1]。

十八烷（500 μg；每天在与母细胞移植相关的外周部位注射；35 天）具有很强的抗癌作用，并显着减缓肿瘤的生长[1]。

细胞毒性测定[1]
细胞类型： B16F10-Nex2 细胞
测试浓度： 12.5、25、50、100 μg/ml
孵育时间： 18 h
实验结果：对B16F10-Nex2细胞表现出较强的细胞毒活性，IC50值为41.08 μg/ml

Animal/Disease Models: C57Bl/6 mice with B16F10-Nex2 Cellsl[1]
Doses: 500 μg
Route of Administration: Injected at peripheral sites in relation to the original cell grafting; daily; 35 days
Experimental Results: Resulted in a significant delay of tumor progression with a significant antitumor effect. The survival rate of treated groups was Dramatically increased.

Absorption, Distribution and Excretion
Samples were collected from the liver, heart, kidneys, muscle, and adipose tissue (perirhinal and subcutaneous) of six cattle for hydrocarbon composition analysis. Qualitative and quantitative analyses were performed using gas chromatography and gas chromatography-mass spectrometry. Despite varying proportions, a series of homologous n-alkanes with carbon chain lengths ranging from n-C12 to n-C31 were found in all samples. Additionally, isoprene hydrocarbons phytane and phyene (phytane-1 and phyene-2) were identified. (These findings are relevant to human health from consuming hydrocarbon-contaminated meat.) /n-Alkanes/ Arthrobacter nana KCC B35, isolated from blue-green cushions covered with oil deposits along the Arabian Gulf coast, was able to grow well using C10 to C40 n-alkanes as its sole carbon and energy source. Growth on C20 to C40 alkanes was even superior to growth on C10 to C18 alkanes. After culturing biomass samples with either n-octacosane (C28) or n-nonacosane (C29) for 6 hours, these compounds became the major alkanes comprising the cellular hydrocarbon component. Even-chain hexadecane (C16) and odd-chain pentadecane (C15) were the second most abundant alkanes in C28 and C29 cultured cells, respectively. Compared to control cells not incubated with hydrocarbon compounds, cells incubated with n-hexadecane accumulated a higher proportion of C16 fatty acids in their lipids. On the other hand, no fatty acids with the same chain length were detected in cells incubated with C28 and C29, but the fatty acid profiles of the cellular lipids suggested that these ultra-long-chain alkanes may have undergone medium-chain oxidation. This activity makes Tobacco Monotyphimurium KCC B35 suitable for use in formulations for the bioremediation of heavy oil deposit contamination.

Toxicity Summary
Identification and Uses: Octacoane is a higher n-alkane containing 28 carbon atoms (C28). Human Exposure and Toxicity: No data are currently available. Animal Studies: A series of homologues of n-alkane, ranging in carbon chain length from n-C12 to n-C31, were found in all samples from bovine liver, heart, kidney, muscle, and adipose tissue.

[1]. Pyrostegia venusta heptane extract containing saturated aliphatic hydrocarbons induces apoptosis on B16F10-Nex2 melanoma cells and displays antitumor activity in vivo. Pharmacogn Mag. 2014 Apr;10(Suppl 2):S363-76.

[2]. Mosquitocidal activities of octacosane from Moschosma polystachyum Linn (lamiaceae). J Ethnopharmacol. 2004 Jan;90(1):87-9.

[3]. Effective targeting of breast cancer cells (MCF7) via novel biogenic synthesis of gold nanoparticles using cancer-derived metabolites. PLoS One. 2020 Oct 6;15(10):e0240156.

waxy hydrocarbon, insoluble in water. Octacosandecane is a straight-chain alkane containing 28 carbon atoms. It is a plant metabolite. Octacosandecane has been reported to exist in Vanilla madagascariensis, Andrachne rotundifolia, and other organisms with relevant data. See also: Moringa leaf oil (partial).

C28H58

394.76

394.454

630-02-4

Octacosane-d58;16416-33-4

12408

White to off-white solid powder

278 °C15 mm Hg(lit.)

57-62 °C(lit.)

227 °C

<1 mm Hg ( 20 °C)

1.4330 (70ºC)

11.168

0

0

0

25

28

218

0

ZYURHZPYMFLWSH-UHFFFAOYSA-N

InChI=1S/C28H58/c1-3-5-7-9-11-13-15-17-19-21-23-25-27-28-26-24-22-20-18-16-14-12-10-8-6-4-2/h3-28H2,1-2H3

octacosane

2934.99.9001

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples

注意: 如下所列的是一些常用的体内动物实验溶解配方，主要用于溶解难溶或不溶于水的产品（水溶度<1 mg/mL）。 建议您先取少量样品进行尝试，如该配方可行，再根据实验需求增加样品量。

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注射用配方1: DMSO : Tween 80： Saline = 10 : 5 : 85 (如： 100 μL DMSO → 50 μL Tween 80 → 850 μL Saline)
*生理盐水/Saline的制备：将0.9g氯化钠/NaCl溶解在100 mL ddH ₂ O中，得到澄清溶液。
注射用配方 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (如： 100 μL DMSO → 400 μL PEG300 → 50 μL Tween 80 → 450 μL Saline)
注射用配方 3: DMSO : Corn oil = 10 : 90 (如： 100 μL DMSO → 900 μL Corn oil)
示例: 以注射用配方 3 (DMSO : Corn oil = 10 : 90) 为例说明, 如果要配制 1 mL 2.5 mg/mL的工作液, 您可以取 100 μL 25 mg/mL 澄清的 DMSO 储备液，加到 900 μL Corn oil/玉米油中, 混合均匀。

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注射用配方 4: DMSO : 20% SBE-β-CD in Saline = 10 : 90 [如：100 μL DMSO → 900 μL (20% SBE-β-CD in Saline)]
*20% SBE-β-CD in Saline的制备（4°C，储存1周）：将2g SBE-β-CD (磺丁基-β-环糊精) 溶解于10mL生理盐水中，得到澄清溶液。
注射用配方 5: 2-Hydroxypropyl-β-cyclodextrin : Saline = 50 : 50 (如： 500 μL 2-Hydroxypropyl-β-cyclodextrin (羟丙基环胡精)→ 500 μL Saline)
注射用配方 6: DMSO : PEG300 : Castor oil : Saline = 5 : 10 : 20 : 65 (如： 50 μL DMSO → 100 μL PEG300 → 200 μL Castor oil → 650 μL Saline)
注射用配方 7: Ethanol : Cremophor : Saline = 10： 10 : 80 (如： 100 μL Ethanol → 100 μL Cremophor → 800 μL Saline)
注射用配方 8: 溶解于Cremophor/Ethanol (50 : 50), 然后用生理盐水稀释。
注射用配方 9: EtOH : Corn oil = 10 : 90 (如： 100 μL EtOH → 900 μL Corn oil)
注射用配方 10: EtOH : PEG300：Tween 80 : Saline = 10 : 40 : 5 : 45 (如： 100 μL EtOH → 400 μL PEG300 → 50 μL Tween 80 → 450 μL Saline)

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口服配方 1: 悬浮于0.5% CMC Na (羧甲基纤维素钠)
口服配方 2: 悬浮于0.5% Carboxymethyl cellulose (羧甲基纤维素)
示例: 以口服配方 1 (悬浮于 0.5% CMC Na)为例说明, 如果要配制 100 mL 2.5 mg/mL 的工作液, 您可以先取0.5g CMC Na并将其溶解于100mL ddH2O中，得到0.5%CMC-Na澄清溶液；然后将250 mg待测化合物加到100 mL前述 0.5%CMC Na溶液中，得到悬浮液。

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口服配方 3: 溶解于 PEG400 (聚乙二醇400)
口服配方 4: 悬浮于0.2% Carboxymethyl cellulose (羧甲基纤维素)
口服配方 5: 溶解于0.25% Tween 80 and 0.5% Carboxymethyl cellulose (羧甲基纤维素)
口服配方 6: 做成粉末与食物混合

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注意: 以上为较为常见方法，仅供参考， InvivoChem并未独立验证这些配方的准确性。具体溶剂的选择首先应参照文献已报道溶解方法、配方或剂型，对于某些尚未有文献报道溶解方法的化合物，需通过前期实验来确定（建议先取少量样品进行尝试），包括产品的溶解情况、梯度设置、动物的耐受性等。

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请根据您的实验动物和给药方式选择适当的溶解配方/方案：
1、请先配制澄清的储备液(如：用DMSO配置50 或 100 mg/mL母液(储备液));
2、取适量母液，按从左到右的顺序依次添加助溶剂，澄清后再加入下一助溶剂。以 下列配方为例说明 (注意此配方只用于说明，并不一定代表此产品 的实际溶解配方):
10% DMSO → 40% PEG300 → 5% Tween-80 → 45% ddH2O (或 saline);
假设最终工作液的体积为 1 mL, 浓度为5 mg/mL: 取 100 μL 50 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀/澄清；向上述体系中加入50 μL Tween-80，混合均匀/澄清；然后继续加入450 μL ddH2O (或 saline)定容至 1 mL；
3、溶剂前显示的百分比是指该溶剂在最终溶液/工作液中的体积所占比例;
4、 如产品在配制过程中出现沉淀/析出，可通过加热(≤50℃)或超声的方式助溶;
5、为保证最佳实验结果，工作液请现配现用！
6、如不确定怎么将母液配置成体内动物实验的工作液，请查看说明书或联系我们;
7、 以上所有助溶剂都可在 Invivochem.cn网站购买。