Molnupiravir (EIDD-2801, MK-4482)

别名: Molnupiravir; MK-4482; MK4482; MK 4482; EIDD-2801; EIDD 2801; EIDD2801; prodrug-EIDD-1931; prodrug-EIDD 1931; prodrug-EIDD1931; molnupiravirum; 莫匹那韦
目录号: V37614 纯度: ≥98%
Molnupiravir(EIDD-2801;MK4482;前药-EIDD1931;MK-4482;商品名Lagevrio)是核糖核苷类似物EIDD-1931的一种新型、有效、口服生物可利用的前药,对流感病毒和多种冠状病毒具有广谱抗病毒活性,例如如 COVID-19、SARS-CoV-2、MERS-CoV、SARS-CoV。
Molnupiravir (EIDD-2801, MK-4482) CAS号: 2349386-89-4
产品类别: SARS-CoV
产品仅用于科学研究,不针对患者销售
规格 价格 库存 数量
10 mM * 1 mL in DMSO
5mg
10mg
25mg
50mg
100mg
250mg
500mg
1g
2g
Other Sizes

Other Forms of Molnupiravir (EIDD-2801, MK-4482):

  • EIDD-1931 (Beta-d-N4-hydroxycytidine; NHC)
  • Molnupiravir (EIDD-2801; MK-4482)
点击了解更多
InvivoChem产品被CNS等顶刊论文引用
纯度/质量控制文件

纯度: ≥98%

纯度: ≥98%

产品描述
Molnupiravir(EIDD-2801;MK4482;前药-EIDD1931;MK-4482;商品名Lagevrio)是核糖核苷类似物EIDD-1931的一种新型、有效、口服生物可利用的前药,对流感病毒和多种冠状病毒具有广谱抗病毒活性,例如 COVID-19、SARS-CoV-2、MERS-CoV、SARS-CoV。 EIDD-2801 具有治疗 COVID-19 以及季节性和大流行性流感的潜力。 2021 年 11 月 4 日,Molnupiravir 在英国获得批准用于对抗 COVID-19 大流行。 EIDD-2801已被证明可以改善肺功能、减少体重减轻并减少肺部病毒数量。除了针对冠状病毒的活性外,EIDD-2801在实验室研究中还显示出针对季节性流感和禽流感、呼吸道合胞病毒、基孔肯雅病毒、埃博拉病毒、委内瑞拉马脑炎病毒和东部马脑炎病毒的活性。
生物活性&实验参考方法
靶点
Broad-spectrum antiviral; RNA-dependent RNA polymerase
体外研究 (In Vitro)
严重急性呼吸综合征冠状病毒2的RNA依赖性RNA聚合酶是当前治疗2019冠状病毒病药物开发工作的重要靶点。莫努匹拉韦是一种广谱抗病毒药物,是核苷类似物β-D-N4-羟基胞苷(NHC)的口服生物可利用前药。Molnupiravir或NHC可以增加复制冠状病毒的G到A和C到U过渡突变。突变频率的增加可能与抗病毒作用的增加有关;然而,莫努匹拉韦诱导突变的生化数据尚未报道。在这里,我们研究了活性化合物NHC5'-三磷酸(NHC-TP)对纯化的严重急性呼吸综合征冠状病毒2 RNA依赖性RNA聚合酶复合物的作用。天然核苷酸的掺入效率高于NHC-TP掺入模型RNA底物的效率,其顺序为GTP(12841)>ATP(424)>UTP(171)>CTP(30),表明NHC-TP主要与CTP竞争掺入。由于在RNA引物链中掺入一磷酸,没有观察到对RNA合成的显著抑制。当嵌入模板链中时,一磷酸NHC以相似的效率支持NHC:G和NHC:A碱基对的形成。NHC:G产物的延伸受到适度抑制,但较高的核苷酸浓度可以克服这种阻碍。相反,NHC:A碱基对导致观察到的G到A(G:NHC:A)或C到U(C:G:NHC:A:U)突变。总之,这些生物化学数据支持莫努匹拉韦的作用机制,该机制主要基于通过模板链介导的RNA诱变[3]。
体内研究 (In Vivo)
莫努匹拉韦是一种强效抗病毒药物,以50–500 mg/kg的剂量每12小时口服一次,持续三天,可以阻止严重急性呼吸系统综合征冠状病毒的复制和疾病[1]
莫努匹拉韦(7 mg/kg;口服;每天两次,持续3.5天)显著降低了病毒的脱落量,缩短了发烧的持续时间[2]。冠状病毒(CoV)在物种之间频繁传播,导致新的疾病爆发,最近的例子是新出现的SARS-CoV-2,新冠肺炎的病原体。在这里,我们发现核糖核苷类似物β-d-N4-羟基胞苷(NHC;EIDD-1931)对严重急性呼吸系统综合征冠状病毒2型、MERS-CoV、严重急性呼吸综合征冠状病毒和相关人畜共患2b或2c组蝙蝠冠状病毒具有广谱抗病毒活性,并对携带核苷类似物抑制剂瑞德西韦耐药性突变的冠状病毒具有更高的效力。在感染严重急性呼吸系统综合征冠状病毒或MERS-CoV的小鼠中,预防和治疗性给予EIDD-2801,一种口服生物可利用的NHC前药(β-d-N4-羟基胞苷-5’-异丙基酯),可以改善肺功能,降低病毒滴度和体重减轻。体外和体内MERS-CoV产量的降低与病毒中过渡突变频率的增加有关,但与宿主细胞RNA无关,这支持了CoV致命突变的机制。NHC/EIDD-2801对抗多种冠状病毒的效力和口服生物利用度突出了其作为对抗严重急性呼吸系统综合征冠状病毒2型和其他未来人畜共患冠状病毒的有效抗病毒药物的潜在效用[1]。
酶活实验
蛋白质的表达和纯化[3]
如上所述,通过使用杆状病毒表达系统将nsp-5、-7、-8和-12表达为多蛋白,并通过在nsp-8 N-末端组氨酸标签上的Ni-NTA亲和层析纯化nsp-7-8-12复合物,产生了严重急性呼吸系统综合征冠状病毒2型RdRp复合物
NTP掺入和包埋引物或模板的NHC-MP对病毒RNA合成的影响[3]
根据我们的报告,通过严重急性呼吸系统综合征冠状病毒2型RdRp掺入NTP,并进行数据采集和定量。单核苷酸和多核苷酸掺入测定的酶浓度分别为100或200 nM。RNA合成孵育时间为10分钟。使用来自单核苷酸掺入测定的数据来确定天然核苷酸相对于NHC-TP的偏好。选择性值计算为天然核苷酸与核苷酸类似物的结合效率之比。核苷酸掺入的效率由Michaelis–Menten常数Vmax与Km的比值决定。核苷酸掺入底物是通过将[α-32P]NTP掺入4-nt引物而产生的5-nt引物。5-nt引物的形成在给定的时间点是最大的;然而,它的确切浓度是未知的。因此,通过量化对应于6-nt引物产物的信号并将其除以反应中的总信号(5-nt引物和6-nt引物)来测量反应中产生的产物。这定义了产品分数。产物分数通常乘以总底物浓度,以确定Vmax的摩尔单位,这在这里是不可能的,如上所述。因此,Vmax的单位被报告为随时间变化的乘积分数。选择性值是无单位的,因为它是具有相同单位的两个Vmax/Km测量的比率。如我们所述,制备了具有嵌入NHC-MP的RNA模板。图S1中解释了与NHC相关的方案修改。
细胞实验
Madin-Darby犬肾(MDCK)细胞(ATCC CCL-34)在补充有7.5%胎牛血清(FBS)的Dulbecco改良Eagle培养基(DMEM)中于37°C和5%CO2下生长。来自30岁健康女性供体的正常原代人支气管气管上皮细胞(HBTECs)在支气管生命细胞培养基中生长。这些细胞由供应商在知情同意的情况下获得,并符合赫尔辛基宣言、《人体组织法》(英国)、CFR第21篇和HIPAA法规。所有监管审批均由供应商负责。本研究中使用的永生细胞系定期检查微生物污染(间隔约6个月)。2017年7月25日,LifeLine Cell Technology对HBTEC进行了微生物污染测试。本研究仅使用了通道编号为1-4的重型作战电子计算机[2]。
动物实验
Animal Model: C57BL/6 mice (intranasal infection with SARS-CoV)[1]
Dosage: 50, 150, 500 mg/kg
Administration: Oral; every 12 hours for 3 days
Result: Body weight loss is significantly diminished or prevented.
PK in cynomolgus macaques[2]
Non-naïve cynomolgus macaques (4 males, 4 females; 2 to 6 years of age) retrieved from the colony stock at Concord Biosciences and originally received from Chares River Laboratories were each dosed orally with 100 mg/kg of NHC dissolved in 240 mM citrate buffer, followed by blood collection from the femoral vein at the specified time points. After a 7-day washout period, each animal was dosed orally with 130 mg/kg of EIDD-2801 dissolved in 80% (v/v) PEG-400, 20% (v/v) N,N-Dimethylacetamide, followed by blood collection from the femoral vein at the specified time points. In a separate study, non-naïve cynomolgus macaques (3 males, 3 females; 2 to 6 years of age) retrieved from the colony stock at MPI, Inc and originally received from Chares River Laboratories were each dosed intravenously with 10 mg/kg of NHC dissolved in 0.9% sterile sodium chloride, followed by blood collection from the femoral vein at the specified time points. For all samples, plasma was separated from heparinized blood and stored at −80°C before analysis as described in NHC. For calibration, standard curves were prepared in blank plasma (concentrations range 10 to 10,000 ng/ml). Quality-control samples of 30, 500, and 5000 ng/ml in blank plasma were analyzed at the beginning of each sample set. Calibration showed linearity with R2 values >0.99.
PK and PD in ferrets[2]
Female ferrets (6 to 8 months of age) received from Marshall BioRescoursces were rested for one week, assigned randomly to study groups and dosed orally with EIDD-2801 dissolved in 1% methylcellulose, followed by blood collection from the anterior vena cava and tissue sampling at the specified time points. Three animals per groups were sampled for PK analyses and 2-3 animals for PD testing. Plasma was separated from heparinized blood, and tissue samples snap-frozen and stored at −80°C before analysis as described in NHC. For calibration, standard curves were prepared in blank plasma (concentrations range 10 to 100,000 ng/ml) and blank tissue lysate (concentration range 1.56 to 3,130 ng/ml), respectively. Quality-control samples of 30, 500 and 5,000 ng/ml in blank plasma were analyzed at the beginning of each sample set. Calibration in each matrix showed linearity with R2 values >0.99.
Influenza infection studies in ferrets[2]
Female ferrets (6 to 8 months of age) were received from Marshall BioRescoursces and housed in an ABSL-2 (animal biosafety level) facility. Ferrets were rested for 1 week, weighed, assigned randomly to groups, anesthetized with dexmedetomidine/ketamine, and infected intranasally with 1 × 105 (A/California/07/2009 (H1N1)) or 1 × 106 pfu (A/Wisconsin/67/2005 (H3N2)); infection volume 200 μl. Treatment with EIDD-2801 was initiated 3 hours before infection (prophylactic regimen), 12 hours post-infection (post-exposure prophylactic regimen), or 24 hours post-infection (therapeutic regimen), and continued for 3.5 days b.i.d. Compound was administered orally in 3.5 ml doses in 1% methylcellulose formulation and chased with 3.5 ml high-calorie liquid dietary supplement. Control groups received vehicle (1% methylcellulose in water) volume equivalents. Body temperature was monitored continuously (readings every 2-15 minutes) using implanted telemetric sensors. Bodyweight of animals was measured at start and end of each experiment, and for some experiments daily; no changes in body weight were detected. Additional monitoring of phenotypically appreciable adverse effects included assessment of animals for changes in overall composure, activity level or vocation and occurrence of diarrhea, vomiting or reduced food uptake. Viral load was measured from nasal lavages (collected in 12-hour intervals) and nasal turbinates (upper respiratory tract), harvested 3.5 days after infection if not specified otherwise.
参考文献

[1]. An orally bioavailable broad-spectrum antiviral inhibits\nSARS-CoV-2 in human airway epithelial cell cultures and multiple\ncoronaviruses in mice. Sci Transl Med. 2020 Apr 6. pii: eabb5883.

[2]. Characterization of orally efficacious influenza drug with\nhigh resistance barrier in ferrets and human airway epithelia. Sci\nTransl Med. 2019 Oct 23;11(515). pii: eaax5866.

[3]. Molnupiravir promotes SARS-CoV-2 mutagenesis via the RNA template. Biol Chem. 2021 Jul; 297(1): 100770.
其他信息
Molnupiravir is a nucleoside analogue that is N(4)-hydroxycytidine in which the 5'-hydroxy group is replaced by a (2-methylpropanoyl)oxy group. It is the prodrug of the active antiviral ribonucleoside analog N(4)-hydroxycytidine (EIDD-1931), has activity against a number of RNA viruses including SARS-CoV-2, MERS-CoV, and seasonal and pandemic influenza viruses. It is currently in phase III trials for the treatment of patients with COVID-19. It has a role as a prodrug, an anticoronaviral agent and an antiviral drug. It is a nucleoside analogue, an isopropyl ester and a ketoxime. It is functionally related to a N(4)-hydroxycytidine. ChEBI
Molnupiravir (EIDD-2801, MK-4482) is the isopropylester prodrug of [N4-hydroxycytidine]. With improved oral bioavailability in non-human primates, it is hydrolyzed in vivo, and distributes into tissues where it becomes the active 5’-triphosphate form. The active drug incorporates into the genome of RNA viruses, leading to an accumulation of mutations known as viral error catastrophe. Recent studies have shown molnupiravir inhibits replication of human and bat coronaviruses, including SARS-CoV-2, in mice and human airway epithelial cells. A [remdesivir] resistant mutant mouse hepatitis virus has also been shown to have increased sensitivity to N4-hydroxycytidine. Molnupiravir was granted approval by the UK's Medicines and Health products Regulatory Agency (MHRA) on 4 November 2021 to prevent severe outcomes such as hospitalization and death due to COVID-19 in adults. Molnupiravir was also granted emergency use authorization by the FDA on December 23, 2021; however, it is not yet fully approved. DrugBank
View More

Molnupiravir is a ribonucleoside analogue and antiviral agent that is used in the therapy the severe acute respiratory syndrome (SARS) coronavirus 2 (CoV-2) infection, the cause of the novel coronavirus disease, 2019 (COVID-19). Molnupiravir therapy is given orally for 5 days early in the course of SARS-CoV-2 infection and has not been linked to serum aminotransferase elevations or to clinically apparent liver injury. LiverTox


Molnupiravir is an orally bioavailable prodrug of EIDD-1931, the synthetic ribonucleoside derivative N4-hydroxycytidine and ribonucleoside analog, with potential antiviral activity against a variety of RNA viruses. Upon oral administration, molnupiravir, being a prodrug, is metabolized into its active form EIDD-1931 and converted into its triphosphate (TP) form. The TP form of EIDD-1931 is incorporated into RNA and inhibits the action of viral RNA-dependent RNA polymerase. This results in the termination of RNA transcription and decreases viral RNA production, and viral RNA replication.
[N4-hydroxycytidine] and its prodrug molnupiravir are being studied for its activity against a number of viral infections including influenza, MERS-CoV, and SARS-CoV-2. Molnupiravir is approved in the UK for reducing the risk of hospitalization and death in mild to moderate COVID-19 cases for patients at increased risk of severe disease (eg. with obesity, diabetes mellitus, heart disease, or are over 60 years old). In the US, molnupiravir is authorized for emergency use for the treatment of high-risk adults With mild to moderate COVID-19.
Molnupiravir is a ribonucleoside analogue and antiviral agent that is used in the therapy the severe acute respiratory syndrome (SARS) coronavirus 2 (CoV-2) infection, the cause of the novel coronavirus disease, 2019 (COVID-19). Molnupiravir therapy is given orally for 5 days early in the course of SARS-CoV-2 infection and has not been linked to serum aminotransferase elevations or to clinically apparent liver injury.
Absorption: After an 800 mg oral dose of molnupiravir every 12 hours, the active compound (N4-hydroxycytidine) reaches a Cmax of 2970 ng/mL, with a Tmax of 1.5 hours, and an AUC0-12h of 8360 h\*ng/mL.
Route of Elimination: ≤3% of an oral molnupiravir dose is eliminated in the urine as the active metabolite N4-hydroxycytidine. DrugBank Metabolism / Metabolites: Molnupiravir is hydrolyzed to [N4-hydroxycytidine], which distributes into tissues. Once inside cells, N4-hydroxycytidine is phosphorylated to the 5'-triphosphate form.
Biological Half-Life: The half life of the active metabolite, N4-hydroxycytidine, is 3.3 hours.
Mechanism of Action: Molnupiravir is hydrolyzed _in vivo_ to N4-hydroxycytidine, which is phosphorylated in tissue to the active 5’-triphosphate form, and incorporated into the genome of new virions, resulting in the accumulation of inactivating mutations, known as viral error catastrophe. A [remdesivir] resistant mutant mouse hepatitis virus has also been shown to have increased sensitivity to N4-hydroxycytidine.
Hepatotoxicity: In preregistration clinical trials, serum aminotransferase elevations were uncommon and mild, and were no more frequent with molnupiravir than with placebo. Furthermore, among more than 900 patients treated with molnupiravir (800 mg twice daily) for 5 days in prelicensure studies, there were no reported episodes of clinically apparent liver injury. Confounding the issue is that serum aminotransferase elevations are common during symptomatic SARS-CoV-2 infection, present in up to 70% of patients and are more frequent in patients with severe disease and in those with the known risk factors for COVID-19 severity such as male sex, older age, higher body mass index and diabetes. Thus, molnupiravir has not been shown to cause liver injury, but the total clinical experience with its use is limited.
◈ What is molnupiravir?
Molnupiravir is an investigational antiviral medication. Investigational (or experimental) drugs are ones that are being studied to see if they work. Molnupiravir is being studied for the treatment of SARS-CoV-2 (which causes COVID-19). Molnupiravir is given by mouth (orally). A brand name for molnupiravir is Lagevrio®. For this medication to be effective, it must be started within 5 days of having symptoms of COVID-19.Because molnupiravir is still being studied, there is limited information about whether or not it is safe and/or effective. However, the U.S. Food and Drug Administration (FDA) gave emergency permission for molnupiravir to be used to treat some patients with mild-to-moderate COVID-19 infection. COVID-19 infection can increase the chance of pregnancy complications. For more information about COVID-19, please the see the MotherToBaby fact sheet at https://mothertobaby.org/fact-sheets/covid-19/.According to the emergency use label, the use of molnupiravir is not recommended during pregnancy based on animal data that suggests a possible concern. However, your healthcare providers can talk with you about the benefits of treating your condition and the risks of untreated illness during pregnancy.
◈ I am taking molnupiravir, but I would like to be finished with taking it before becoming pregnant. How long does the drug stay in my body?
People eliminate medication at different rates. In non-pregnant adults, it takes up to 1 day, on average, for most of the molnupiravir to be gone from the body. However, it is recommended by the emergency use label that females avoid trying to get pregnant during the time they are taking molnupiravir and for 4 days after the last dose of molnupiravir.
◈ I take molnupiravir. Can it make it harder for me to get pregnant?
Studies have not been done to see if molnupiravir can make it harder to get pregnant. It is recommended by the emergency use label that females who can get pregnant use effective contraception correctly and consistently while they are taking molnupiravir and for 4 days after the last dose of molnupiravir.
◈ Does taking molnupiravir increase the chance for miscarriage?
Miscarriage can occur in any pregnancy. Animal studies suggested an increased chance for miscarriage. Studies have not been done in humans to see if molnupiravir increases the chance for miscarriage. It is not known whether COVID-19 infection itself increases the chance of miscarriage.
◈ Does taking molnupiravir increase the chance of birth defects?
Every pregnancy starts out with a 3-5% chance of having a birth defect. This is called the background risk. Studies have not been done in humans to see if molnupiravir does or does not increase the chance for birth defects above the background risk. Animal studies by the manufacturer suggest an increase in birth defects when molnupiravir was given at 8 times the human dose. These birth defects involved the eyes, kidneys, and some bones. It is not known whether COVID-19 infection can increase the chance of birth defects.
◈ Does taking molnupiravir in pregnancy increase the chance of other pregnancy-related problems?
Studies have not been done in humans to see if molnupiravir increases the chance for pregnancy-related problems such as preterm delivery (birth before week 37) or low birth weight (weighing less than 5 pounds, 8 ounces [2500 grams] at birth). An animal study reported lower fetal weight and a lower amount of mineralized bone (delayed ossification) when molnupiravir was given at 3 times the human dose. At this dose, other effects on fetal development, miscarriage, or stillbirth were not seen.There is evidence to suggest that COVID-19 infection increases the chance of stillbirth or the mother dying during childbirth. Other negative pregnancy outcomes that appear to be related to COVID-19 include spontaneous preterm delivery, fetal growth restriction, and bleeding in the mother after birth (postpartum hemorrhage).
◈ Does taking molnupiravir in pregnancy affect future behavior or learning for the child?
Studies have not been done to see if molnupiravir can cause behavior or learning issues for the child.
◈ Breastfeeding while taking molnupiravir:
The emergency use label for molnupiravir recommends people who are breastfeeding not use this medication. But, the benefit of using molnupiravir while breastfeeding may outweigh possible risks. People who are breastfeeding may consider pumping and discarding breast milk during treatment with molnupiravir and for 4 days after the last dose. Your healthcare providers can talk with you about using molnupiravir and what treatment is best for you and your baby. Be sure to talk to your healthcare provider about all of your breastfeeding questions.
◈ If a male takes molnupiravir, could it affect fertility (ability to get partner pregnant) or increase the chance of birth defects?
Studies have not been done to see if molnupiravir could affect male fertility or increase the chance of birth defects. The product label notes that, while the risk is considered to be low, it is recommended that males use a reliable method of contraception correctly and consistently during treatment and for at least 3 months after the last dose of molnupiravir. In general, exposures that fathers or sperm donors have are unlikely to increase the risks to a pregnancy. For more information, please see the MotherToBaby fact sheet Paternal Exposures at https://mothertobaby.org/fact-sheets/paternal-exposures-pregnancy/.

*注: 文献方法仅供参考, InvivoChem并未独立验证这些方法的准确性
化学信息 & 存储运输条件
分子式
C13H19N3O7
分子量
329.3059
精确质量
329.12
元素分析
C, 47.42; H, 5.82; N, 12.76; O, 34.01
CAS号
2349386-89-4
相关CAS号
3258-02-4 (EIDD-1931);2492423-29-5
PubChem CID
145996610
外观&性状
White to off-white solid powder
LogP
-0.8
tPSA
141Ų
SMILES
O[C@@H]([C@H]([C@H](N1C(N/C(C=C1)=N/O)=O)O2)O)[C@H]2COC(C(C)C)=O
InChi Key
HTNPEHXGEKVIHG-QCNRFFRDSA-N
InChi Code
InChI=1S/C13H19N3O7/c1-6(2)12(19)22-5-7-9(17)10(18)11(23-7)16-4-3-8(15-21)14-13(16)20/h3-4,6-7,9-11,17-18,21H,5H2,1-2H3,(H,14,15,20)/t7-,9-,10-,11-/m1/s1
化学名
((2R,3S,4R,5R)-3,4-dihydroxy-5-((E)-4-(hydroxyimino)-2-oxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl)methyl isobutyrate
别名
Molnupiravir; MK-4482; MK4482; MK 4482; EIDD-2801; EIDD 2801; EIDD2801; prodrug-EIDD-1931; prodrug-EIDD 1931; prodrug-EIDD1931; molnupiravirum;
HS Tariff Code
2934.99.9001
存储方式

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

运输条件
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
溶解度数据
溶解度 (体外实验)
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
溶解度 (体内实验)
注意: 如下所列的是一些常用的体内动物实验溶解配方,主要用于溶解难溶或不溶于水的产品(水溶度<1 mg/mL)。 建议您先取少量样品进行尝试,如该配方可行,再根据实验需求增加样品量。

注射用配方
(IP/IV/IM/SC等)
注射用配方1: DMSO : Tween 80: Saline = 10 : 5 : 85 (如: 100 μL DMSO 50 μL Tween 80 850 μL Saline)
*生理盐水/Saline的制备:将0.9g氯化钠/NaCl溶解在100 mL ddH ₂ O中,得到澄清溶液。
注射用配方 2: DMSO : PEG300Tween 80 : Saline = 10 : 40 : 5 : 45 (如: 100 μL DMSO 400 μL PEG300 50 μL Tween 80 450 μL Saline)
注射用配方 3: DMSO : Corn oil = 10 : 90 (如: 100 μL DMSO 900 μL Corn oil)
示例: 注射用配方 3 (DMSO : Corn oil = 10 : 90) 为例说明, 如果要配制 1 mL 2.5 mg/mL的工作液, 您可以取 100 μL 25 mg/mL 澄清的 DMSO 储备液,加到 900 μL Corn oil/玉米油中, 混合均匀。
View More

注射用配方 4: DMSO : 20% SBE-β-CD in Saline = 10 : 90 [如:100 μL DMSO 900 μL (20% SBE-β-CD in Saline)]
*20% SBE-β-CD in Saline的制备(4°C,储存1周):将2g SBE-β-CD (磺丁基-β-环糊精) 溶解于10mL生理盐水中,得到澄清溶液。
注射用配方 5: 2-Hydroxypropyl-β-cyclodextrin : Saline = 50 : 50 (如: 500 μL 2-Hydroxypropyl-β-cyclodextrin (羟丙基环胡精) 500 μL Saline)
注射用配方 6: DMSO : PEG300 : Castor oil : Saline = 5 : 10 : 20 : 65 (如: 50 μL DMSO 100 μL PEG300 200 μL Castor oil 650 μL Saline)
注射用配方 7: Ethanol : Cremophor : Saline = 10: 10 : 80 (如: 100 μL Ethanol 100 μL Cremophor 800 μL Saline)
注射用配方 8: 溶解于Cremophor/Ethanol (50 : 50), 然后用生理盐水稀释。
注射用配方 9: EtOH : Corn oil = 10 : 90 (如: 100 μL EtOH 900 μL Corn oil)
注射用配方 10: EtOH : PEG300Tween 80 : Saline = 10 : 40 : 5 : 45 (如: 100 μL EtOH 400 μL PEG300 50 μL Tween 80 450 μL Saline)


口服配方
口服配方 1: 悬浮于0.5% CMC Na (羧甲基纤维素钠)
口服配方 2: 悬浮于0.5% Carboxymethyl cellulose (羧甲基纤维素)
示例: 口服配方 1 (悬浮于 0.5% CMC Na)为例说明, 如果要配制 100 mL 2.5 mg/mL 的工作液, 您可以先取0.5g CMC Na并将其溶解于100mL ddH2O中,得到0.5%CMC-Na澄清溶液;然后将250 mg待测化合物加到100 mL前述 0.5%CMC Na溶液中,得到悬浮液。
View More

口服配方 3: 溶解于 PEG400 (聚乙二醇400)
口服配方 4: 悬浮于0.2% Carboxymethyl cellulose (羧甲基纤维素)
口服配方 5: 溶解于0.25% Tween 80 and 0.5% Carboxymethyl cellulose (羧甲基纤维素)
口服配方 6: 做成粉末与食物混合


注意: 以上为较为常见方法,仅供参考, InvivoChem并未独立验证这些配方的准确性。具体溶剂的选择首先应参照文献已报道溶解方法、配方或剂型,对于某些尚未有文献报道溶解方法的化合物,需通过前期实验来确定(建议先取少量样品进行尝试),包括产品的溶解情况、梯度设置、动物的耐受性等。

请根据您的实验动物和给药方式选择适当的溶解配方/方案:
1、请先配制澄清的储备液(如:用DMSO配置50 或 100 mg/mL母液(储备液));
2、取适量母液,按从左到右的顺序依次添加助溶剂,澄清后再加入下一助溶剂。以 下列配方为例说明 (注意此配方只用于说明,并不一定代表此产品 的实际溶解配方):
10% DMSO → 40% PEG300 → 5% Tween-80 → 45% ddH2O (或 saline);
假设最终工作液的体积为 1 mL, 浓度为5 mg/mL: 取 100 μL 50 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀/澄清;向上述体系中加入50 μL Tween-80,混合均匀/澄清;然后继续加入450 μL ddH2O (或 saline)定容至 1 mL;

3、溶剂前显示的百分比是指该溶剂在最终溶液/工作液中的体积所占比例;
4、 如产品在配制过程中出现沉淀/析出,可通过加热(≤50℃)或超声的方式助溶;
5、为保证最佳实验结果,工作液请现配现用!
6、如不确定怎么将母液配置成体内动物实验的工作液,请查看说明书或联系我们;
7、 以上所有助溶剂都可在 Invivochem.cn网站购买。
制备储备液 1 mg 5 mg 10 mg
1 mM 3.0367 mL 15.1833 mL 30.3665 mL
5 mM 0.6073 mL 3.0367 mL 6.0733 mL
10 mM 0.3037 mL 1.5183 mL 3.0367 mL

1、根据实验需要选择合适的溶剂配制储备液 (母液):对于大多数产品,InvivoChem推荐用DMSO配置母液 (比如:5、10、20mM或者10、20、50 mg/mL浓度),个别水溶性高的产品可直接溶于水。产品在DMSO 、水或其他溶剂中的具体溶解度详见上”溶解度 (体外)”部分;

2、如果您找不到您想要的溶解度信息,或者很难将产品溶解在溶液中,请联系我们;

3、建议使用下列计算器进行相关计算(摩尔浓度计算器、稀释计算器、分子量计算器、重组计算器等);

4、母液配好之后,将其分装到常规用量,并储存在-20°C或-80°C,尽量减少反复冻融循环。

计算器

摩尔浓度计算器可计算特定溶液所需的质量、体积/浓度,具体如下:

  • 计算制备已知体积和浓度的溶液所需的化合物的质量
  • 计算将已知质量的化合物溶解到所需浓度所需的溶液体积
  • 计算特定体积中已知质量的化合物产生的溶液的浓度
使用摩尔浓度计算器计算摩尔浓度的示例如下所示:
假如化合物的分子量为350.26 g/mol,在5mL DMSO中制备10mM储备液所需的化合物的质量是多少?
  • 在分子量(MW)框中输入350.26
  • 在“浓度”框中输入10,然后选择正确的单位(mM)
  • 在“体积”框中输入5,然后选择正确的单位(mL)
  • 单击“计算”按钮
  • 答案17.513 mg出现在“质量”框中。以类似的方式,您可以计算体积和浓度。

稀释计算器可计算如何稀释已知浓度的储备液。例如,可以输入C1、C2和V2来计算V1,具体如下:

制备25毫升25μM溶液需要多少体积的10 mM储备溶液?
使用方程式C1V1=C2V2,其中C1=10mM,C2=25μM,V2=25 ml,V1未知:
  • 在C1框中输入10,然后选择正确的单位(mM)
  • 在C2框中输入25,然后选择正确的单位(μM)
  • 在V2框中输入25,然后选择正确的单位(mL)
  • 单击“计算”按钮
  • 答案62.5μL(0.1 ml)出现在V1框中
g/mol

分子量计算器可计算化合物的分子量 (摩尔质量)和元素组成,具体如下:

注:化学分子式大小写敏感:C12H18N3O4  c12h18n3o4
计算化合物摩尔质量(分子量)的说明:
  • 要计算化合物的分子量 (摩尔质量),请输入化学/分子式,然后单击“计算”按钮。
分子质量、分子量、摩尔质量和摩尔量的定义:
  • 分子质量(或分子量)是一种物质的一个分子的质量,用统一的原子质量单位(u)表示。(1u等于碳-12中一个原子质量的1/12)
  • 摩尔质量(摩尔重量)是一摩尔物质的质量,以g/mol表示。
/

配液计算器可计算将特定质量的产品配成特定浓度所需的溶剂体积 (配液体积)

  • 输入试剂的质量、所需的配液浓度以及正确的单位
  • 单击“计算”按钮
  • 答案显示在体积框中
动物体内实验配方计算器(澄清溶液)
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
第二步:请输入动物体内配方组成(配方适用于不溶/难溶于水的化合物),不同的产品和批次配方组成不同,如对配方有疑问,可先联系我们提供正确的体内实验配方。此外,请注意这只是一个配方计算器,而不是特定产品的确切配方。
+
+
+

计算结果:

工作液浓度 mg/mL;

DMSO母液配制方法 mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL)。如该浓度超过该批次药物DMSO溶解度,请首先与我们联系。

体内配方配制方法μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL ddH2O,混匀澄清。

(1) 请确保溶液澄清之后,再加入下一种溶剂 (助溶剂) 。可利用涡旋、超声或水浴加热等方法助溶;
            (2) 一定要按顺序加入溶剂 (助溶剂) 。

临床试验信息
Efficacy and Safety of Molnupiravir in Healthy Participants Inoculated With Experimental Influenza Virus (MK-4482-019)
CTID: NCT05818124
Phase: Phase 1
Status: Completed
Date: 2024-07-11
Assessing Antiviral Treatments in Early Symptomatic RSV
CTID: NCT06488300
Phase: Phase 2
Status: Not yet recruiting
Date: 2024-07-11
A Study to Evaluate Molnupiravir (MK-4482; MOV) in Participants With Severe Renal Impairment (MK-4482-003)
CTID: NCT05386758
Phase: Phase 1
Status: Completed
Date: 2024-07-05
Finding Treatments for COVID-19: A Trial of Antiviral Pharmacodynamics in Early Symptomatic COVID-19 (PLATCOV)
CTID: NCT05041907
Phase: Phase 2
Status: Recruiting
Date: 2024-06-27
A Phase 2 Trial Comparing Antiviral Treatments in Early Symptomatic Influenza
CTID: NCT05648448
Phase: Phase 2
Status: Recruiting
Date: 2024-06-06
生物数据图片
  • NHC potently inhibits MERS-CoV and newly emerging SARS-CoV-2 replication. [1].Sci Transl Med. 2020 Apr 6. pii: eabb5883.
  • NHC is highly active against SARS-CoV-2, MERS-CoV, and SARS-CoV in primary human airway epithelial cell cultures. [1].Sci Transl Med. 2020 Apr 6. pii: eabb5883.
  • Remdesivir (RDV) resistance mutations in the highly conserved RNA-dependent RNA polymerase increase susceptibility to NHC. [1].Sci Transl Med. 2020 Apr 6. pii: eabb5883.
  • NHC is effective against multiple genetically distinct Bat-CoV.[1].Sci Transl Med. 2020 Apr 6. pii: eabb5883.
  • NHC antiviral activity is associated with increased viral mutation rates. [1].Sci Transl Med. 2020 Apr 6. pii: eabb5883.
  • Prophylactic and therapeutic EIDD-2801 reduces SARS-CoV replication and pathogenesis.[1].Sci Transl Med. 2020 Apr 6. pii: eabb5883.
  • Prophylactic and therapeutic EIDD-2801 reduces MERS-CoV replication and pathogenesis coincident with increased viral mutation rates. [1].Sci Transl Med. 2020 Apr 6. pii: eabb5883.
相关产品
联系我们